Detailed information of TA system TA204236

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 226827..227465
• Replicon: chromosome
• Accession: NZ_CP075697
• Organism: Escherichia coli strain STEC2018-607

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: JFX52_RS01115
• Protein ID: WP_000813794.1
• Coordinates: 227289..227465 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JFX52_RS01110
• Protein ID: WP_077878996.1
• Coordinates: 226827..227243 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX52_RS01090 (221979)	221979..222920	-	942	WP_001251315.1	ABC transporter permease	-
JFX52_RS01095 (222921)	222921..223934	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
JFX52_RS01100 (223952)	223952..225097	-	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
JFX52_RS01105 (225342)	225342..226748	-	1407	WP_032331455.1	PLP-dependent aminotransferase family protein	-
JFX52_RS01110 (226827)	226827..227243	-	417	WP_077878996.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
JFX52_RS01115 (227289)	227289..227465	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
JFX52_RS01120 (227687)	227687..227917	+	231	WP_000494244.1	YncJ family protein	-
JFX52_RS01125 (228009)	228009..229970	-	1962	WP_024178299.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
JFX52_RS01130 (230043)	230043..230579	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
JFX52_RS01135 (230671)	230671..231846	+	1176	WP_001236258.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-67)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T204236 WP_000813794.1 NZ_CP075697:c227465-227289 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T204236 NZ_CP100678:c4228304-4227813 [Salmonella enterica subsp. enterica serovar Anatum]
ATGATCTCCACCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATAGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGACCACTGGCGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAACGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCTCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGGTTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTTGGTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAAGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTACCAGCGGGTGGGGTTTGTACCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15260.66 Da
• Isoelectric Point: 4.7485

>AT204236 WP_077878996.1 NZ_CP075697:c227243-226827 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVEIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT204236 NZ_CP100678:c4228594-4228301 [Salmonella enterica subsp. enterica serovar Anatum]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGTGAAACTCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATTGA
TCGAGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAAGCTGCCCGTGCTGCCGCAGAAGAAGCGCTTA
TCGAACAACGCATCATTATGGCCGATCCAGAGGCTTATCAGGAATTTCTCGTTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA

Structures

Toxin

• PDB: 6HPB