Detailed information of TA system TA204233

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE-Phd
• Location: 11100..11711
• Replicon: plasmid pPAL8-03
• Accession: NZ_CP075689
• Organism: Pseudomonas amygdali pv. lachrymans strain 8

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: A0A3M3DN15
• Locus tag: C4C37_RS28515
• Protein ID: WP_005746121.1
• Coordinates: 11361..11711 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: A0A3M5ISD9
• Locus tag: C4C37_RS28510
• Protein ID: WP_005746122.1
• Coordinates: 11100..11348 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4C37_RS28490 (C4C37_28490)	6197..7300	+	1104	WP_004663854.1	IS5-like element ISPsy19 family transposase	-
C4C37_RS28495 (C4C37_28495)	7533..8933	+	1401	WP_052508578.1	TrbI/VirB10 family protein	-
C4C37_RS28505 (C4C37_28505)	10323..10850	-	528	WP_227024935.1	hypothetical protein	-
C4C37_RS28510 (C4C37_28510)	11100..11348	+	249	WP_005746122.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
C4C37_RS28515 (C4C37_28515)	11361..11711	+	351	WP_005746121.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4C37_RS28520 (C4C37_28520)	11880..12368	-	489	WP_005746120.1	hypothetical protein	-
C4C37_RS28525 (C4C37_28525)	12578..12694	+	117	Protein_13	prevent-host-death protein	-
C4C37_RS28530 (C4C37_28530)	12742..13416	-	675	Protein_14	IS3 family transposase	-
C4C37_RS28535 (C4C37_28535)	13559..14536	-	978	WP_007247761.1	IS5 family transposase	-
C4C37_RS28540 (C4C37_28540)	14604..14870	-	267	WP_227024934.1	hypothetical protein	-
C4C37_RS28545 (C4C37_28545)	15044..15310	+	267	WP_004642394.1	hypothetical protein	-
C4C37_RS28550 (C4C37_28550)	15307..15927	+	621	WP_004642393.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..49000	49000
	-	inside	IScluster/Tn	-	-	2211..29258	27047

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-101)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12844.78 Da
• Isoelectric Point: 4.5514

>T204233 WP_005746121.1 NZ_CP075689:11361-11711 [Pseudomonas amygdali pv. lachrymans]
MTTFALRFTEVAQQSLEDQVEHLAVTQGFSSAARRIDSLIDAIQDKLLSTPLGYPVSPQLSELGVLHYRELNTDGYRIFY
EVMDSGGITGIVVLLVLGGKQSVEQALIRYCLLQLI

Nucleotide

• Length: 351 bp

>T204233 NZ_CP100678:3408485-3408703 [Salmonella enterica subsp. enterica serovar Anatum]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9405.72 Da
• Isoelectric Point: 9.5019

>AT204233 WP_005746122.1 NZ_CP075689:11100-11348 [Pseudomonas amygdali pv. lachrymans]
MRVETISYLKRNAADLPLDEPLVVTQNGVPAYVVESYADRKRRDESIALVKLLAISSREYSQGKHCSTDELKARLSRRFD
HK

Nucleotide

• Length: 249 bp

>AT204233 NZ_CP100678:3408083-3408457 [Salmonella enterica subsp. enterica serovar Anatum]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A3M3DN15

Antitoxin

• AlphaFold DB: A0A3M5ISD9