Detailed information of TA system TA204232

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 39947..40416
• Replicon: plasmid pPAL8-02
• Accession: NZ_CP075688
• Organism: Pseudomonas amygdali pv. lachrymans strain 8

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q48BA0
• Locus tag: C4C37_RS28270
• Protein ID: WP_002556029.1
• Coordinates: 39947..40228 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q48BA1
• Locus tag: C4C37_RS28275
• Protein ID: WP_002556028.1
• Coordinates: 40228..40416 (-)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4C37_RS28250 (C4C37_28250)	36360..36722	-	363	Protein_30	integrase core domain-containing protein	-
C4C37_RS28255 (C4C37_28255)	36816..37025	+	210	WP_225620208.1	hypothetical protein	-
C4C37_RS28260 (C4C37_28260)	37112..38089	+	978	WP_007247761.1	IS5 family transposase	-
C4C37_RS28265 (C4C37_28265)	38386..39528	+	1143	WP_005746165.1	IQ calmodulin-binding motif-containing protein	-
C4C37_RS28270 (C4C37_28270)	39947..40228	-	282	WP_002556029.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4C37_RS28275 (C4C37_28275)	40228..40416	-	189	WP_002556028.1	hypothetical protein	Antitoxin
C4C37_RS28280 (C4C37_28280)	40605..41240	-	636	WP_054068142.1	recombinase family protein	-
C4C37_RS28285 (C4C37_28285)	41563..42195	+	633	WP_003381804.1	ParA family protein	-
C4C37_RS28290 (C4C37_28290)	42231..42485	+	255	WP_003381801.1	hypothetical protein	-
C4C37_RS28295 (C4C37_28295)	42910..43575	-	666	WP_004667059.1	LexA family transcriptional regulator	-
C4C37_RS28300 (C4C37_28300)	43663..43893	+	231	WP_032704469.1	Cro/CI family transcriptional regulator	-
C4C37_RS28305 (C4C37_28305)	44243..44932	+	690	WP_005746161.1	lytic transglycosylase domain-containing protein	-
C4C37_RS28310 (C4C37_28310)	44929..45267	+	339	WP_017279422.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..72398	72398

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10675.35 Da
• Isoelectric Point: 8.4392

>T204232 WP_002556029.1 NZ_CP075688:c40228-39947 [Pseudomonas amygdali pv. lachrymans]
MLPVVWLSPALDDLREIATYIAWENPSAARRLKSLLQEAIEPVAEHPYLYRSGRAPGTRELVAHPNYVLVYRVTLKRIEV
VNVIHARQEYPSP

Nucleotide

• Length: 282 bp

>T204232 NZ_CP100678:3265416-3265766 [Salmonella enterica subsp. enterica serovar Anatum]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 7043.86 Da
• Isoelectric Point: 4.2550

>AT204232 WP_002556028.1 NZ_CP075688:c40416-40228 [Pseudomonas amygdali pv. lachrymans]
MANLSPIVSEFETDEQAASYDRWFRAKVAASLADPRPAIPHDEVMAEIEALIDQVETQREKR

Nucleotide

• Length: 189 bp

>AT204232 NZ_CP100678:3265771-3266073 [Salmonella enterica subsp. enterica serovar Anatum]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0M9HVA1

Antitoxin

• AlphaFold DB: A0A2G9KWB9