Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RelE(toxin)
Location 5189..5636 Replicon plasmid pPAL8-01
Accession NZ_CP075687
Organism Pseudomonas amygdali pv. lachrymans strain 8

Toxin (Protein)


Gene name relE Uniprot ID A0A3M4C5F0
Locus tag C4C37_RS27690 Protein ID WP_003348606.1
Coordinates 5189..5461 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0P9N5D7
Locus tag C4C37_RS27695 Protein ID WP_004666945.1
Coordinates 5451..5636 (-) Length 62 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C4C37_RS27665 (C4C37_27665) 198..1511 + 1314 WP_214551396.1 replication initiation protein -
C4C37_RS27670 (C4C37_27670) 1737..2024 + 288 WP_004667956.1 HU family DNA-binding protein -
C4C37_RS27675 (C4C37_27675) 2169..2435 + 267 WP_235774981.1 hypothetical protein -
C4C37_RS27680 (C4C37_27680) 2608..2910 - 303 Protein_3 transposase -
C4C37_RS27685 (C4C37_27685) 3520..4962 - 1443 WP_214551397.1 hypothetical protein -
C4C37_RS27690 (C4C37_27690) 5189..5461 - 273 WP_003348606.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C4C37_RS27695 (C4C37_27695) 5451..5636 - 186 WP_004666945.1 hypothetical protein Antitoxin
C4C37_RS27700 (C4C37_27700) 5687..5965 - 279 WP_054068781.1 hypothetical protein -
C4C37_RS29115 6060..6167 - 108 Protein_8 resolvase -
C4C37_RS27705 (C4C37_27705) 6480..6818 - 339 Protein_9 transposase -
C4C37_RS27710 (C4C37_27710) 6925..7320 + 396 WP_005622000.1 CesT family type III secretion system chaperone -
C4C37_RS27715 (C4C37_27715) 7333..8175 + 843 WP_080956113.1 AvrPphF family type III effector -
C4C37_RS27720 (C4C37_27720) 8451..9608 - 1158 WP_005740916.1 putative DNA binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..77748 77748
- inside IScluster/Tn - - 2608..6791 4183


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-89)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10199.67 Da        Isoelectric Point: 5.0749

>T204231 WP_003348606.1 NZ_CP075687:c5461-5189 [Pseudomonas amygdali pv. lachrymans]
VLVEWLPQALEALEEIAGYLAERNPYAAEQMQATLEATVEALPLHPYIHRPGRVPGTREAVVHPNYLVVYRVGTERISIL
DVLHARQEYP

Download         Length: 273 bp

>T204231 NZ_CP100678:2294104-2294388 [Salmonella enterica subsp. enterica serovar Anatum]
ATGACTTATAAGCTGGCATTTAACGAATCTGCACTAAAAGAGTGGAAAAAGCTGGGACATACTATACAGGAACAGTTTAA
AAAAAAGTTACGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGACTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGCGTTGGAAAG
CGTGAAAATGATGCCATCTACAAAGTGACACGACACAGAAGCTAA

Antitoxin


Download         Length: 62 a.a.        Molecular weight: 7311.22 Da        Isoelectric Point: 8.7721

>AT204231 WP_004666945.1 NZ_CP075687:c5636-5451 [Pseudomonas amygdali pv. lachrymans]
MSIQLDPRVSEFETQEQADNYDRWFRQRIERSLADPRPPVPHDEAMARVRAMIEAKRRRAS

Download         Length: 186 bp

>AT204231 NZ_CP100678:2293866-2294114 [Salmonella enterica subsp. enterica serovar Anatum]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACTGAACTGAAACGTGACCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGTAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3M4C5F0

Antitoxin

Source ID Structure
AlphaFold DB A0A0P9N5D7

References