Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 18588..19195 Replicon plasmid pSTEC2018_553-c
Accession NZ_CP075668
Organism Escherichia coli strain STEC2018-553

Toxin (Protein)


Gene name doc Uniprot ID A0A085NWP2
Locus tag JFY30_RS28260 Protein ID WP_000673981.1
Coordinates 18588..18974 (-) Length 129 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID B7LIH9
Locus tag JFY30_RS28265 Protein ID WP_000493532.1
Coordinates 18971..19195 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFY30_RS28240 (13871) 13871..15039 + 1169 Protein_17 TonB-dependent receptor -
JFY30_RS28245 (15108) 15108..16283 + 1176 WP_001020415.1 enterotoxin production-related protein TieB -
JFY30_RS28250 (16913) 16913..17371 - 459 Protein_19 IS110 family transposase -
JFY30_RS28255 (17652) 17652..18349 + 698 WP_094096600.1 IS1-like element IS1A family transposase -
JFY30_RS28260 (18588) 18588..18974 - 387 WP_000673981.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
JFY30_RS28265 (18971) 18971..19195 - 225 WP_000493532.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
JFY30_RS28270 (19261) 19261..19616 + 356 Protein_23 Tn3 family transposase -
JFY30_RS28275 (19850) 19850..19981 - 132 WP_072023375.1 colicin 10 lysis protein -
JFY30_RS28280 (20006) 20006..20296 + 291 WP_215744939.1 Cki family colicin immunity protein -
JFY30_RS28285 (20433) 20433..20881 + 449 Protein_26 colicin immunity protein Cui -
JFY30_RS28290 (20904) 20904..22400 - 1497 WP_198789129.1 colicin-like pore-forming protein -
JFY30_RS28295 (22640) 22640..22930 + 291 WP_000047032.1 hypothetical protein -
JFY30_RS28300 (22922) 22922..23200 - 279 WP_000710675.1 hypothetical protein -
JFY30_RS28305 (23322) 23322..23744 + 423 WP_021539380.1 Exc2 family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - senB 1..36145 36145
- inside IScluster/Tn - senB 9673..17927 8254


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-52)


Sequences

Toxin        


Download         Length: 129 a.a.        Molecular weight: 14416.71 Da        Isoelectric Point: 7.0732

>T204216 WP_000673981.1 NZ_CP075668:c18974-18588 [Escherichia coli]
MKFVSSQEMIEFHDRLISRDGGVAGMPEPGRADAIIHRVLNMYHYEGVTDIMDLAAVYLVAIARGHIFNDANKRTALFVA
QVFLKRNGIHIISSRISFDEMQIIALNAATGEYNWKMVSDHLKAIILN

Download         Length: 387 bp

>T204216 NZ_CP100670:c2499465-2499362 [Salmonella enterica subsp. enterica serovar Mbandaka]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8361.39 Da        Isoelectric Point: 5.6713

>AT204216 WP_000493532.1 NZ_CP075668:c19195-18971 [Escherichia coli]
MFTCNYSHARDNLASLLELAVDENQPVEIQRRKGGAAVLVDKDAFERYRKAALDAEFAEIMQSHGTAYTELSKR

Download         Length: 225 bp

>AT204216 NZ_CP100670:2499360-2499503 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAAGTTTTCCAGTTTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A085NWP2

Antitoxin

Source ID Structure
AlphaFold DB A0A086V9Y8

References