Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 11472..12031 Replicon plasmid pSTEC2018_553-c
Accession NZ_CP075668
Organism Escherichia coli strain STEC2018-553

Toxin (Protein)


Gene name relE Uniprot ID A0A234XMQ9
Locus tag JFY30_RS28225 Protein ID WP_000421251.1
Coordinates 11756..12031 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A2T3SXK7
Locus tag JFY30_RS28220 Protein ID WP_024166087.1
Coordinates 11472..11756 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFY30_RS28205 (7104) 7104..9485 - 2382 Protein_10 conjugative transfer relaxase/helicase TraI -
JFY30_RS28210 (9673) 9673..10860 - 1188 WP_198779709.1 IS91 family transposase -
JFY30_RS28215 (10860) 10860..11225 - 366 WP_000091308.1 hypothetical protein -
JFY30_RS28220 (11472) 11472..11756 + 285 WP_024166087.1 ribbon-helix-helix domain-containing protein Antitoxin
JFY30_RS28225 (11756) 11756..12031 + 276 WP_000421251.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JFY30_RS28230 (12313) 12313..13500 - 1188 WP_198779709.1 IS91 family transposase -
JFY30_RS28235 (13500) 13500..13865 - 366 WP_000091308.1 hypothetical protein -
JFY30_RS28240 (13871) 13871..15039 + 1169 Protein_17 TonB-dependent receptor -
JFY30_RS28245 (15108) 15108..16283 + 1176 WP_001020415.1 enterotoxin production-related protein TieB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - senB 1..36145 36145
- inside IScluster/Tn - senB 9673..17927 8254


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10791.59 Da        Isoelectric Point: 9.9324

>T204215 WP_000421251.1 NZ_CP075668:11756-12031 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARMVQSLIQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T204215 NZ_CP100670:2492156-2492467 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATGCATGTCATATCAAAAGAGCCTTTTGATGAAGCGGCAAGACGATATCCCAATGACTCGTTAGCCATAAAAGCCCTGTA
TCGCTTAGTCCGCGAAAGAGATTTCTCTTCTCCGGCAGAGCTACGAAAAGTCATACCGAGTCTCGATAACTTTAAGTACC
GTAACAAGTGGTGGGTACTGGATGTCGGAGGCAACAACCTGAGGGTGATCGCTTACATCAACTTTATAAACAAACGCTTT
TATGTGAAACACATCGCCACTCACGCTGAATATGACAAGCTGACCCGCTACTACAGGGAGAATAAAGAATGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10482.94 Da        Isoelectric Point: 5.6558

>AT204215 WP_024166087.1 NZ_CP075668:11472-11756 [Escherichia coli]
IQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVISGQIIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT204215 NZ_CP100670:2492464-2492883 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATGATTGCCAACACCGCAAAAGCTATCGAAGCCACAAAGGCGCTGGTCGCCGCCGTTCCTTTCCTGGGTGGCAGCGCGTC
AGAGAAAGATTATCGTGATGCGCTAGCGCTGGTTGATTATCTTATCGAAAATGACGATGAAAACCCGCTGATTGACTTCC
TGGCAAGCAAAATTGCCGAGTACGAAGATAACAGCAAGCAGTTCGCCGAGTTCAACAAATCTGTTGCTGAAATGCCGGTT
GGTGTTGCCCTGCTGCGCACGCTAATTGACCAGTACAAACTCTCATACTCAGATCTTAAAGAAGAAATCGGTTCCAAATC
ACTGGTAAGTCAGATCCTGTCCGGACAGCGCTCGTTAACGATTACGCACATCAAAGCGCTTTCAGCACGATTTGGCGTGA
AACCTGAGTGGTTCTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A234XMQ9

Antitoxin

Source ID Structure
AlphaFold DB A0A2T3SXK7

References