Detailed information of TA system TA204208

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2971..3596
• Replicon: plasmid pSTEC2018_553-b
• Accession: NZ_CP075667
• Organism: Escherichia coli strain STEC2018-553

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JFY30_RS27890
• Protein ID: WP_000911313.1
• Coordinates: 3198..3596 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: JFY30_RS27885
• Protein ID: WP_000450520.1
• Coordinates: 2971..3198 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFY30_RS27885 (JFY30_27885)	2971..3198	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
JFY30_RS27890 (JFY30_27890)	3198..3596	+	399	WP_000911313.1	type II toxin-antitoxin system VapC family toxin	Toxin
JFY30_RS27895 (JFY30_27895)	3605..5758	-	2154	WP_053879280.1	type IV conjugative transfer system coupling protein TraD	-
JFY30_RS27900 (JFY30_27900)	6010..6741	-	732	WP_000850423.1	conjugal transfer complement resistance protein TraT	-
JFY30_RS27905 (JFY30_27905)	6755..7264	-	510	WP_000628105.1	conjugal transfer entry exclusion protein TraS	-
JFY30_RS27910 (JFY30_27910)	7261..8229	-	969	Protein_6	conjugal transfer protein TraG	-
JFY30_RS27915 (JFY30_27915)	8227..8475	-	249	Protein_7	type-F conjugative transfer system secretin TraK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	sitABCD	iutA / iucD / iucC / iucB / iucB / iucA	1..48758	48758

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T204208 WP_000911313.1 NZ_CP075667:3198-3596 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T204208 NZ_CP100670:c449896-449480 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATGAGTAAAACCTACATGCTGGATACCTGCATCTGTTCTTTCATTATGCGCGAACAGCCGGAAGCTGTGCTGAAACGCCT
TGAGCAGGCAGTGCTGCGCCGCCATCGTATTGTGGTGTCGGCGATTACCTACGCCGAAATGCGTTTTGGCTGTACGGGCA
AAAAGGCCTCGCCGCGCCATGCCCAACTGGTTGATGCATTCTGTAGTCGCCTGGATGCCGTGCTGGCCTGGGACCGGGCG
GCAGTGGATGCCACCACAGAGATCCGTGCCGTACTGGCCGCCGTCGGTACGCCGATTGGATCAAACGATGCGGCTATCGC
CGGCCATGCCATTGCGTCCGGGGCGATACTGGTCACTAATAACGTGAGGGAGTTTGAGCGGGTACCGGGTTTGCAGTACG
AAGATTGGGTAAAATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT204208 WP_000450520.1 NZ_CP075667:2971-3198 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT204208 NZ_CP100670:c450123-449893 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATGCGAACCGTATCTGTATTTAAAAATGGCAATAACCGCGCGATCCGCCTTCCACGCGATCTGGATTTTGACGGCGTCAA
CGAACTTGAAATCTTCCGCGAAGGCGACACGATTATACTGCGCCCCGCCCGCCCTTCCTGGGGATCATTCCGTCATGAGG
AAAAAGCCAGCCCAGACTTTCTGACGGAACGCGAAGACATCATCAGCGATGAGGGACGCTTTGAACTATGA

Structures

Antitoxin

• AlphaFold DB: V0VCB2