Detailed information of TA system TA204206

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 14023..14545
• Replicon: plasmid pSTEC2018_553-a
• Accession: NZ_CP075666
• Organism: Escherichia coli strain STEC2018-553

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: JFY30_RS27190
• Protein ID: WP_000220566.1
• Coordinates: 14264..14545 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: JFY30_RS27185
• Protein ID: WP_000121744.1
• Coordinates: 14023..14274 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFY30_RS27160 (JFY30_27160)	10111..10496	+	386	Protein_11	hypothetical protein	-
JFY30_RS27165 (JFY30_27165)	10496..10600	+	105	Protein_12	ROK family protein	-
JFY30_RS27170 (JFY30_27170)	10744..11457	+	714	WP_024178317.1	oligogalacturonate-specific porin KdgM family protein	-
JFY30_RS27175 (JFY30_27175)	11477..12583	+	1107	WP_001044131.1	N-acetylneuraminate epimerase	-
JFY30_RS27180 (JFY30_27180)	12630..12830	-	201	Protein_15	DNA-binding protein	-
JFY30_RS27185 (JFY30_27185)	14023..14274	+	252	WP_000121744.1	hypothetical protein	Antitoxin
JFY30_RS27190 (JFY30_27190)	14264..14545	+	282	WP_000220566.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JFY30_RS27195 (JFY30_27195)	14799..15986	-	1188	WP_198779709.1	IS91 family transposase	-
JFY30_RS27200 (JFY30_27200)	15986..16351	-	366	WP_000091308.1	hypothetical protein	-
JFY30_RS27205 (JFY30_27205)	16559..16744	-	186	WP_001368543.1	hypothetical protein	-
JFY30_RS27210 (JFY30_27210)	16985..17851	-	867	WP_000589629.1	DMT family transporter	-
JFY30_RS27215 (JFY30_27215)	17844..18026	-	183	WP_001368563.1	hypothetical protein	-
JFY30_RS27220 (JFY30_27220)	18066..18266	-	201	WP_000762569.1	hypothetical protein	-
JFY30_RS27225 (JFY30_27225)	18424..18642	+	219	Protein_24	DUF6404 family protein	-
JFY30_RS27230 (JFY30_27230)	18660..19250	-	591	WP_062891001.1	hypothetical protein	-
JFY30_RS27235 (JFY30_27235)	19250..19507	-	258	WP_000371887.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..129935	129935
	-	flank	IS/Tn	-	-	14799..15986	1187

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-85)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11070.88 Da
• Isoelectric Point: 10.5430

>T204206 WP_000220566.1 NZ_CP075666:14264-14545 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLSERLENPRVPASRLHGHADRYKIKLRTSGYRLVYQVIDEKVVLLVISVGK
REGNEVYQIADMR

Nucleotide

• Length: 282 bp

>T204206 NZ_CP100669:c5963-5448 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGAAATTTTACACAATATCAAGCAGTTACATAAAATACCTGAAGGACTTTGATGACAAAGTTCCCAATAGCGAAGATCC
TACATACAACAATCCTAAGGCTTTCATTGGCATAGTACTAGAGATTGAAGGACATAAATATTTAGCACCTTTAACATCGC
CAAAAGCATGGCATGCTAATGTAAAAGAGTCATCACCCGCCTTCTTCAAACTTCATGAAAACGGTGTCCCTGATAATCAG
CTTGGATTGATTAATCTGAAATTTATGATTCCAATAATCGAAGCTGAAGTGTCTTTACTTGATCTGGATAGCATGCCTGA
TACGCCTTACAAAAGAATGCTATATAAACAGTTACAATTTATTCGTGTAAATGAAGATAAAATATCTGAAAAATCAAAAC
TATTAAGAAACCTTGCCTTGCAAGGCAGAATGCAAAGAACATGCGACTTTGCCGTGTTAGAAGAAAAATATCAACATTTC
GGTAAAAAACCTGAAGACATGGAAATAGACGATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9222.48 Da
• Isoelectric Point: 4.1683

>AT204206 WP_000121744.1 NZ_CP075666:14023-14274 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVVILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Nucleotide

• Length: 252 bp

>AT204206 NZ_CP100669:c6080-5965 [Salmonella enterica subsp. enterica serovar Enteritidis]
AGAAATTCAGGTGATTTGCTACCTTTAAGTGCAGCTAGAAATTTAGGTCATTTACTACCTTAAAGTAATAAAGTAAAAAA
GGCGACTATACACAGTCGCCTTTTTTACGAGAAAAA