Detailed information of TA system TA204205

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 1832..2367
• Replicon: plasmid pSTEC2018_553-a
• Accession: NZ_CP075666
• Organism: Escherichia coli strain STEC2018-553

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V0VJJ7
• Locus tag: JFY30_RS27115
• Protein ID: WP_000222760.1
• Coordinates: 2080..2367 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0VIP2
• Locus tag: JFY30_RS27110
• Protein ID: WP_001132900.1
• Coordinates: 1832..2083 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFY30_RS27105 (JFY30_27105)	1..873	+	873	WP_000729440.1	incFII family plasmid replication initiator RepA	-
JFY30_RS28940	1568..1759	+	192	WP_033810767.1	hypothetical protein	-
JFY30_RS27110 (JFY30_27110)	1832..2083	+	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JFY30_RS27115 (JFY30_27115)	2080..2367	+	288	WP_000222760.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JFY30_RS27120 (JFY30_27120)	2471..3061	+	591	WP_000977350.1	serine acetyltransferase	-
JFY30_RS27125 (JFY30_27125)	3164..3820	+	657	WP_000536048.1	ProQ/FINO family protein	-
JFY30_RS27130 (JFY30_27130)	3888..4202	+	315	WP_000633742.1	hypothetical protein	-
JFY30_RS27140 (JFY30_27140)	4971..6152	-	1182	Protein_7	IS91 family transposase	-
JFY30_RS27145 (JFY30_27145)	6640..7180	-	541	Protein_8	GntR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..129935	129935

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11105.15 Da
• Isoelectric Point: 10.5832

>T204205 WP_000222760.1 NZ_CP075666:2080-2367 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKSIQQQFAKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Nucleotide

• Length: 288 bp

>T204205 NZ_CP100668:c28031-27750 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9211.41 Da
• Isoelectric Point: 4.0780

>AT204205 WP_001132900.1 NZ_CP075666:1832-2083 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT204205 NZ_CP100668:c28272-28021 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• AlphaFold DB: V0VJJ7

Antitoxin

• AlphaFold DB: V0VIP2