Detailed information of TA system TA204204

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5293341..5293966
• Replicon: chromosome
• Accession: NZ_CP075665
• Organism: Escherichia coli strain STEC2018-553

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JFY30_RS26275
• Protein ID: WP_000911330.1
• Coordinates: 5293568..5293966 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: JFY30_RS26270
• Protein ID: WP_000450524.1
• Coordinates: 5293341..5293568 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFY30_RS26245 (5289144)	5289144..5289614	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
JFY30_RS26250 (5289614)	5289614..5290186	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
JFY30_RS26255 (5290332)	5290332..5291210	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
JFY30_RS26260 (5291227)	5291227..5292261	+	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
JFY30_RS26265 (5292474)	5292474..5293187	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
JFY30_RS26270 (5293341)	5293341..5293568	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
JFY30_RS26275 (5293568)	5293568..5293966	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
JFY30_RS26280 (5294113)	5294113..5294976	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
JFY30_RS26285 (5294991)	5294991..5297006	+	2016	WP_000829394.1	tRNA cytosine(34) acetyltransferase TmcA	-
JFY30_RS26290 (5297080)	5297080..5297778	+	699	WP_000679812.1	esterase	-
JFY30_RS26295 (5297888)	5297888..5298088	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T204204 WP_000911330.1 NZ_CP075665:5293568-5293966 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T204204 NZ_CP100667:c61624-61319 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCAGTTTAAGGTTTACACCTGTAAAAGGGAGAGTCGCTACCGGCTGTTTGTGGACGTGCAGAGCGACATCATTGACAC
CCCCGGACGGCGGATGGCTGTCCCGCTGGTCAGCGCCCGTCTGCTGTCGGAAAAGGTTCCCCGCGATCTTTACCCGGTGA
TGCATATCGGGGATGAGCCTTACCGCCTGCTGACCACGGATATGACCAGTGTGCCGGCCACCGTCATCGGGGAAGAGGTG
GCCGATCTCAGCCTCCGGGAGAACGATATCAAAAACGCCATTAACCTGATGTTCCGGGGGATCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT204204 WP_000450524.1 NZ_CP075665:5293341-5293568 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT204204 NZ_CP100667:c61844-61626 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGAAGCAGCGAATTACAGTGACAGTGGACAGCGACAGCTATCAGCTGCTCAAGGCATACGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGCCGTCTGCGAGCCGAACGCTGGCAGGAAGAAAATCGGGAAGGTATGGCTG
AGGTGGCCAGCTTTATAGAGGCTAACGGATCGTTTGCTGACGACAACAGGAACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33