Detailed information of TA system TA204197

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 3310573..3311159
• Replicon: chromosome
• Accession: NZ_CP075665
• Organism: Escherichia coli strain STEC2018-553

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0H0RQZ4
• Locus tag: JFY30_RS16650
• Protein ID: WP_042014162.1
• Coordinates: 3310929..3311159 (-)
• Length: 77 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JFY30_RS16645
• Protein ID: WP_000593552.1
• Coordinates: 3310573..3310932 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFY30_RS16620 (3306705)	3306705..3307649	+	945	WP_000947068.1	nickel ABC transporter permease subunit NikB	-
JFY30_RS16625 (3307646)	3307646..3308479	+	834	WP_001008963.1	nickel ABC transporter permease subunit NikC	-
JFY30_RS16630 (3308479)	3308479..3309243	+	765	WP_001136236.1	nickel import ATP-binding protein NikD	-
JFY30_RS16635 (3309240)	3309240..3310046	+	807	WP_000173666.1	nickel import ATP-binding protein NikE	-
JFY30_RS16640 (3310052)	3310052..3310453	+	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
JFY30_RS16645 (3310573)	3310573..3310932	-	360	WP_000593552.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
JFY30_RS16650 (3310929)	3310929..3311159	-	231	WP_042014162.1	type II toxin-antitoxin system HicA family toxin	Toxin
JFY30_RS16655 (3311263)	3311263..3312387	-	1125	WP_001216257.1	ABC transporter permease	-
JFY30_RS16660 (3312387)	3312387..3315122	-	2736	WP_000149132.1	ribosome-associated ATPase/putative transporter RbbA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (15-65)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 77 a.a.
• Molecular weight: 8419.79 Da
• Isoelectric Point: 8.5518

>T204197 WP_042014162.1 NZ_CP075665:c3311159-3310929 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Nucleotide

• Length: 231 bp

>T204197 NZ_CP100666:c3663728-3663315 [Salmonella enterica subsp. enterica serovar Enteritidis]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTAA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13462.37 Da
• Isoelectric Point: 5.1987

>AT204197 WP_000593552.1 NZ_CP075665:c3310932-3310573 [Escherichia coli]
MIKLKTPNFMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT204197 NZ_CP100666:c3663975-3663709 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGACGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0H0RQZ4