Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2613152..2613806 Replicon chromosome
Accession NZ_CP075665
Organism Escherichia coli strain STEC2018-553

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag JFY30_RS12980 Protein ID WP_000244777.1
Coordinates 2613152..2613559 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JFY30_RS12985 Protein ID WP_000354046.1
Coordinates 2613540..2613806 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFY30_RS12960 (2609109) 2609109..2610842 - 1734 WP_000813168.1 single-stranded-DNA-specific exonuclease RecJ -
JFY30_RS12965 (2610848) 2610848..2611558 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JFY30_RS12970 (2611583) 2611583..2612479 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JFY30_RS12975 (2612591) 2612591..2613112 + 522 WP_001055874.1 flavodoxin FldB -
JFY30_RS12980 (2613152) 2613152..2613559 - 408 WP_000244777.1 protein YgfX Toxin
JFY30_RS12985 (2613540) 2613540..2613806 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JFY30_RS12990 (2614049) 2614049..2615029 + 981 WP_000886063.1 tRNA-modifying protein YgfZ -
JFY30_RS12995 (2615225) 2615225..2615884 - 660 WP_000250274.1 hemolysin III family protein -
JFY30_RS13000 (2616048) 2616048..2616359 - 312 WP_001182947.1 N(4)-acetylcytidine aminohydrolase -
JFY30_RS13005 (2616404) 2616404..2617837 + 1434 WP_024178269.1 6-phospho-beta-glucosidase BglA -
JFY30_RS13010 (2617894) 2617894..2618637 - 744 WP_024178268.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T204196 WP_000244777.1 NZ_CP075665:c2613559-2613152 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T204196 NZ_CP100666:c3652824-3652426 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT204196 WP_000354046.1 NZ_CP075665:c2613806-2613540 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT204196 NZ_CP100666:c3653051-3652824 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGTAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References