204194

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 2292881..2293718 Replicon chromosome
Accession NZ_CP075665
Organism Escherichia coli strain STEC2018-553

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag JFY30_RS11420 Protein ID WP_000227784.1
Coordinates 2293176..2293718 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag JFY30_RS11415 Protein ID WP_001297137.1
Coordinates 2292881..2293192 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFY30_RS11390 (2287901) 2287901..2288848 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
JFY30_RS11395 (2288870) 2288870..2290861 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
JFY30_RS11400 (2290851) 2290851..2291465 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
JFY30_RS11405 (2291465) 2291465..2291794 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
JFY30_RS11410 (2291806) 2291806..2292696 + 891 WP_000971336.1 heme o synthase -
JFY30_RS11415 (2292881) 2292881..2293192 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
JFY30_RS11420 (2293176) 2293176..2293718 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
JFY30_RS11425 (2293774) 2293774..2294709 - 936 WP_001368479.1 tetratricopeptide repeat protein -
JFY30_RS11430 (2295117) 2295117..2296481 + 1365 WP_001000974.1 MFS transporter -
JFY30_RS11435 (2296609) 2296609..2297100 - 492 WP_001138904.1 nucleotide binding protein YajQ -
JFY30_RS11440 (2297268) 2297268..2298179 + 912 WP_000705874.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T204194 WP_000227784.1 NZ_CP075665:2293176-2293718 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T204194 NZ_CP100666:2044865-2045020 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT204194 WP_001297137.1 NZ_CP075665:2292881-2293192 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT204194 NZ_CP100666:c2044853-2044795 [Salmonella enterica subsp. enterica serovar Enteritidis]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGCAAAGCATGAAATTGGCCTCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References