Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2258802..2259420 Replicon chromosome
Accession NZ_CP075665
Organism Escherichia coli strain STEC2018-553

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JFY30_RS11250 Protein ID WP_001291435.1
Coordinates 2259202..2259420 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JFY30_RS11245 Protein ID WP_000344800.1
Coordinates 2258802..2259176 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFY30_RS11235 (2253891) 2253891..2255084 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JFY30_RS11240 (2255107) 2255107..2258256 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
JFY30_RS11245 (2258802) 2258802..2259176 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JFY30_RS11250 (2259202) 2259202..2259420 + 219 WP_001291435.1 HHA domain-containing protein Toxin
JFY30_RS11255 (2259592) 2259592..2260143 + 552 WP_000102564.1 maltose O-acetyltransferase -
JFY30_RS11260 (2260259) 2260259..2260729 + 471 WP_000136192.1 YlaC family protein -
JFY30_RS11265 (2260893) 2260893..2262443 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JFY30_RS11270 (2262485) 2262485..2262838 - 354 WP_000878141.1 DUF1428 family protein -
JFY30_RS11280 (2263217) 2263217..2263528 + 312 WP_000409911.1 MGMT family protein -
JFY30_RS11285 (2263559) 2263559..2264131 - 573 WP_000779827.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T204193 WP_001291435.1 NZ_CP075665:2259202-2259420 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T204193 NZ_CP100666:1795397-1795500 [Salmonella enterica subsp. enterica serovar Enteritidis]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT204193 WP_000344800.1 NZ_CP075665:2258802-2259176 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT204193 NZ_CP100666:c1795502-1795357 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References