Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) hicAB/-
Location 1642521..1643110 Replicon chromosome
Accession NZ_CP075665
Organism Escherichia coli strain STEC2018-553

Toxin (Protein)


Gene name hicA Uniprot ID A0A398TMA1
Locus tag JFY30_RS08280 Protein ID WP_000513533.1
Coordinates 1642862..1643110 (-) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A3J8R0E8
Locus tag JFY30_RS08275 Protein ID WP_000681611.1
Coordinates 1642521..1642865 (-) Length 115 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFY30_RS08240 (1638972) 1638972..1639349 - 378 WP_001271433.1 hypothetical protein -
JFY30_RS28685 (1639557) 1639557..1639826 - 270 WP_001115081.1 hypothetical protein -
JFY30_RS08250 (1639881) 1639881..1640084 - 204 WP_001240876.1 Cro/CI family transcriptional regulator -
JFY30_RS08255 (1640180) 1640180..1640893 + 714 WP_001056249.1 LexA family transcriptional regulator -
JFY30_RS08260 (1640990) 1640990..1641151 + 162 WP_071525586.1 transmembrane anchored protein -
JFY30_RS08265 (1641331) 1641331..1642233 + 903 WP_000081325.1 serine dehydrogenasease -
JFY30_RS08270 (1642234) 1642234..1642404 + 171 WP_167580584.1 hypothetical protein -
JFY30_RS08275 (1642521) 1642521..1642865 - 345 WP_000681611.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
JFY30_RS08280 (1642862) 1642862..1643110 - 249 WP_000513533.1 type II toxin-antitoxin system HicA family toxin Toxin
JFY30_RS08285 (1643874) 1643874..1644644 + 771 WP_000529245.1 Rha family transcriptional regulator -
JFY30_RS08290 (1644715) 1644715..1644927 + 213 WP_000917251.1 hypothetical protein -
JFY30_RS08295 (1644939) 1644939..1645220 + 282 WP_000934192.1 hypothetical protein -
JFY30_RS08300 (1645241) 1645241..1645522 + 282 WP_000995348.1 host nuclease inhibitor GamL -
JFY30_RS08305 (1645540) 1645540..1646490 + 951 WP_000459722.1 RecT family recombinase -
JFY30_RS08310 (1646487) 1646487..1647176 + 690 WP_000187063.1 YqaJ viral recombinase family protein -
JFY30_RS08315 (1647176) 1647176..1647763 + 588 WP_033805714.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - stxB / stxA 1590134..1661093 70959


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(20-81)

Antitoxin

(54-104)


Sequences


Toxin        


Download         Length: 83 a.a.        Molecular weight: 9436.07 Da        Isoelectric Point: 10.3195

>T204192 WP_000513533.1 NZ_CP075665:c1643110-1642862 [Escherichia coli]
MGKADKLLEKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL

Download         Length: 249 bp

>T204192 NZ_CP100666:c1076891-1076673 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 115 a.a.        Molecular weight: 13065.80 Da        Isoelectric Point: 4.6791

>AT204192 WP_000681611.1 NZ_CP075665:c1642865-1642521 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEASTLAELEQEFKTSVELYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVNHHTGA

Download         Length: 345 bp

>AT204192 NZ_CP100666:c1077293-1076919 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A398TMA1


Antitoxin

Source ID Structure
AlphaFold DB A0A3J8R0E8

References