Detailed information of TA system TA204191

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1132219..1132857
• Replicon: chromosome
• Accession: NZ_CP075665
• Organism: Escherichia coli strain STEC2018-553

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: JFY30_RS05615
• Protein ID: WP_000813794.1
• Coordinates: 1132681..1132857 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JFY30_RS05610
• Protein ID: WP_001270286.1
• Coordinates: 1132219..1132635 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFY30_RS05590 (1127372)	1127372..1128312	-	941	Protein_1110	ABC transporter permease	-
JFY30_RS05595 (1128313)	1128313..1129326	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
JFY30_RS05600 (1129344)	1129344..1130489	-	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
JFY30_RS05605 (1130734)	1130734..1132140	-	1407	WP_000760592.1	PLP-dependent aminotransferase family protein	-
JFY30_RS05610 (1132219)	1132219..1132635	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
JFY30_RS05615 (1132681)	1132681..1132857	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
JFY30_RS05620 (1133079)	1133079..1133309	+	231	WP_000494244.1	YncJ family protein	-
JFY30_RS05625 (1133401)	1133401..1135362	-	1962	WP_024178299.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
JFY30_RS05630 (1135435)	1135435..1135971	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
JFY30_RS05635 (1136063)	1136063..1137244	+	1182	WP_032308624.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1137284..1138549	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T204191 WP_000813794.1 NZ_CP075665:c1132857-1132681 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T204191 NZ_CP100666:c886529-886272 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGAATGCCTCCGGCGTCAGCGTCCGCCACATCAACAGCAAAACCCACATGACCACCTATTACTCACAAATCCCCAGCCT
GCATCTTAAGGGCGACTGGCTGGAAGAAGCGGGATTTAAGACCGGGCGCGGCGTCACCGTGAAGATTTCACAGGGGTGTA
TTGTGCTGATGGCGGACAGTAACGAGGAGCAGAAGCTGCGCGAGCAGCTTTATCAGGCTAAACAGGTGGTTAAGGGAATT
AAGGACGTGCTGGTTTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT204191 WP_001270286.1 NZ_CP075665:c1132635-1132219 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT204191 NZ_CP100666:886354-886686 [Salmonella enterica subsp. enterica serovar Enteritidis]
TCCGCCATCAGCACAATACACCCCTGTGAAATCTTCACGGTGACGCCGCGCCCGGTCTTAAATCCCGCTTCTTCCAGCCA
GTCGCCCTTAAGATGCAGGCTGGGGATTTGTGAGTAATAGGTGGTCATGTGGGTTTTGCTGTTGATGTGGCGGACGCTGA
CGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTAATTATCCGCCGGATGCTGGCTGATTGTGGAGCTCAGGGTG
AGCGAGTATGGGCGCGACATCCTGGCACTGAGCCCCCCTCTCCCCGGCCAGCTCCGGGCGGTGATGAGCAACCGGCTGCC
GGGGCCGTATTTT

Structures

Toxin

• PDB: 6HPB