Detailed information of TA system TA204180

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 51472..52097
• Replicon: plasmid pSTEC17_197-a
• Accession: NZ_CP075664
• Organism: Escherichia coli strain STEC2017-197

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JFX55_RS24615
• Protein ID: WP_000911317.1
• Coordinates: 51699..52097 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L4J1D2
• Locus tag: JFX55_RS24610
• Protein ID: WP_000450532.1
• Coordinates: 51472..51699 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX55_RS24610 (51472)	51472..51699	+	228	WP_000450532.1	toxin-antitoxin system antitoxin VapB	Antitoxin
JFX55_RS24615 (51699)	51699..52097	+	399	WP_000911317.1	type II toxin-antitoxin system VapC family toxin	Toxin
JFX55_RS24620 (52106)	52106..52738	-	633	Protein_53	type IV secretion system DNA-binding domain-containing protein	-
JFX55_RS24625 (52801)	52801..53957	+	1157	WP_085948462.1	IS3-like element IS600 family transposase	-
JFX55_RS24630 (54004)	54004..55155	+	1152	WP_072158966.1	conjugal transfer protein TraW	-
JFX55_RS24635 (55152)	55152..55721	+	570	WP_000650755.1	conjugal transfer protein TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyA / hlyC	1..118523	118523

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14857.14 Da
• Isoelectric Point: 8.5264

>T204180 WP_000911317.1 NZ_CP075664:51699-52097 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Nucleotide

• Length: 399 bp

>T204180 NZ_CP075664:51699-52097 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGTGTCAGGGAACGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACTCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGACGTTCTGGATTACGACGCTGCTGCTGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAATTGAAGACTGGAGTTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8512.55 Da
• Isoelectric Point: 4.5358

>AT204180 WP_000450532.1 NZ_CP075664:51472-51699 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT204180 NZ_CP075664:51472-51699 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCTGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCTGCCGGAGAGACATGGGACGAATGGTTCGACGGAA
ACAGTGTCAGCGCTGATTTTATGGATAACAGGGAACAGCCTGGTATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CHF5