Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 4296878..4297461 Replicon chromosome
Accession NZ_CP075663
Organism Escherichia coli strain STEC2017-197

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag JFX55_RS20960 Protein ID WP_000254745.1
Coordinates 4297126..4297461 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag JFX55_RS20955 Protein ID WP_000581937.1
Coordinates 4296878..4297126 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX55_RS20945 (4293217) 4293217..4294518 + 1302 WP_000046791.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
JFX55_RS20950 (4294566) 4294566..4296800 + 2235 WP_000226815.1 GTP pyrophosphokinase -
JFX55_RS20955 (4296878) 4296878..4297126 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
JFX55_RS20960 (4297126) 4297126..4297461 + 336 WP_000254745.1 endoribonuclease MazF Toxin
JFX55_RS20965 (4297532) 4297532..4298323 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
JFX55_RS20970 (4298551) 4298551..4300188 + 1638 WP_198790181.1 CTP synthase (glutamine hydrolyzing) -
JFX55_RS20975 (4300276) 4300276..4301574 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T204174 WP_000254745.1 NZ_CP075663:4297126-4297461 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T204174 NZ_CP100653:c1596503-1596321 [Pseudomonas aeruginosa]
ATGAAGTTCAGCGAATTCAGACGATGGTTGAAGGCCCAAGGGGTGACCTTCGAAGCCGGCAAGGGAAGCCACTTCAAGAT
CACCGCCCCGAACGGCAAACAGACCACCTTCGCGGACCACGGAGCTAAGGAAATGCCAGAACCGACCCGCAAGGCGATCA
TCAAGCAACTGGGGCTCAAATGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT204174 WP_000581937.1 NZ_CP075663:4296878-4297126 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT204174 NZ_CP100653:c1596274-1595846 [Pseudomonas aeruginosa]
ATGTACGACTATGCAATCCGTTTCGAACAGGACGATAGCGCTCCTGGCGTCGCCGTTTTCTGCAGAGACCTGCCGGAACT
GAACAGCTACGGCGACGATAAGGCCCATGCAATCAGCGAGGCGGTTGACGCCATCGAGTCAACCCTCTCGCTGTACGTTG
ATCAGCGCCGAGAAATCCCCTCAGCCAGCCAGGCACAACCAGGCGAGCGCGTTATCCATCTGCCGGCGGTTACCGTTGCA
AAGGTCGCGCTCTGGAACGAAATGATCCGTCGAGATATGCGAAAAGCTGACCTCTGCCGGCTCCTCGGGATCGCGCAGAT
CCAGGGTGACAGGCTCGTCGACTTCCTCCACAACACCAAGATGGAAGCCATGGAGAACGCGCTATCCGCCATTGGCCTCC
GCCTCTCAGTGAATATCGAGGTGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References