Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4160595..4161249 Replicon chromosome
Accession NZ_CP075663
Organism Escherichia coli strain STEC2017-197

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag JFX55_RS20345 Protein ID WP_000244777.1
Coordinates 4160842..4161249 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JFX55_RS20340 Protein ID WP_000354046.1
Coordinates 4160595..4160861 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX55_RS20315 (4155764) 4155764..4156507 + 744 WP_000951964.1 SDR family oxidoreductase -
JFX55_RS20320 (4156564) 4156564..4157997 - 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
JFX55_RS20325 (4158042) 4158042..4158353 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
JFX55_RS20330 (4158517) 4158517..4159176 + 660 WP_000250274.1 hemolysin III family protein -
JFX55_RS20335 (4159372) 4159372..4160352 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
JFX55_RS20340 (4160595) 4160595..4160861 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JFX55_RS20345 (4160842) 4160842..4161249 + 408 WP_000244777.1 protein YgfX Toxin
JFX55_RS20350 (4161289) 4161289..4161810 - 522 WP_001055874.1 flavodoxin FldB -
JFX55_RS20355 (4161922) 4161922..4162818 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JFX55_RS20360 (4162843) 4162843..4163553 + 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JFX55_RS20365 (4163559) 4163559..4165292 + 1734 WP_000813223.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T204173 WP_000244777.1 NZ_CP075663:4160842-4161249 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T204173 NZ_CP100653:1207771-1208085 [Pseudomonas aeruginosa]
ATGATCTTCATCGAAACGCCAGTCTTCACGAAGCGGATTCTCGCGCTGGTAGACGACGAGACCTATCGCAAGCTTCAAGA
AGACCTAACGCTCCACCCGGACGCCGGAGTGGTCATTGAAGGCACGGGTGGCGTGCGCAAGATTCGCATTGCTGCCAATG
GCCACGGGAAAAGAGGCGGCGCCCGGGTCATCTACTACCATTTCACATCCGCCTCTCAAATTGCATTCCTGCTGGTCTAT
GACAAGGCATCGCAGGAAGACCTGACTGCCGATCAGAAAAAGGTGCTTCGGCAGATCGTTGAGAACTGGAGATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT204173 WP_000354046.1 NZ_CP075663:4160595-4160861 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT204173 NZ_CP100653:1208088-1208393 [Pseudomonas aeruginosa]
ATGGACAAAGAACTCTTCGCTGAACTGGTCGAAAGCGTGACCCAGATGGACGAGATCGTGCGTGGCGAACGTCAACCTTC
CCGCGAGTTCCACGTCGACGCGGTGAAGGTCAAGGAAATCCGCCGCGCTACTGGCCTGTCCCAGGCCAAATTCGCCCGAA
AGATCGATGTTGCTGTTGGAACCCTGCGCAATTGGGAGCAAGGGCGCCGCGATCCCGAGGGGCCTGCCCGCGCCCTGCTC
CGGGCCATTGACAATGATCCGGTTCATGTTCTGGCTGCCCTGAATGGTGGCCCGCGAGAAAGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References