Detailed information of TA system TA204172

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4004686..4005484
• Replicon: chromosome
• Accession: NZ_CP075663
• Organism: Escherichia coli strain STEC2017-197

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: JFX55_RS19600
• Protein ID: WP_000854902.1
• Coordinates: 4004686..4005063 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A093DMQ4
• Locus tag: JFX55_RS19605
• Protein ID: WP_001280952.1
• Coordinates: 4005110..4005484 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX55_RS19570 (4000032)	4000032..4001009	+	978	WP_000633193.1	type II secretion system minor pseudopilin GspK	-
JFX55_RS19575 (4001006)	4001006..4002184	+	1179	WP_000094987.1	type II secretion system protein GspL	-
JFX55_RS19580 (4002186)	4002186..4002722	+	537	WP_000942798.1	GspM family type II secretion system protein YghD	-
JFX55_RS19585 (4003065)	4003065..4003907	-	843	WP_001280537.1	DUF4942 domain-containing protein	-
JFX55_RS19590 (4003992)	4003992..4004189	-	198	WP_000839261.1	DUF957 domain-containing protein	-
JFX55_RS19595 (4004201)	4004201..4004689	-	489	WP_000779175.1	DUF5983 family protein	-
JFX55_RS19600 (4004686)	4004686..4005063	-	378	WP_000854902.1	TA system toxin CbtA family protein	Toxin
JFX55_RS19605 (4005110)	4005110..4005484	-	375	WP_001280952.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JFX55_RS19610 (4005647)	4005647..4005868	-	222	WP_000692312.1	DUF987 domain-containing protein	-
JFX55_RS19615 (4005931)	4005931..4006407	-	477	WP_001186725.1	RadC family protein	-
JFX55_RS19620 (4006423)	4006423..4006908	-	486	WP_000849590.1	antirestriction protein	-
JFX55_RS19625 (4006963)	4006963..4007781	-	819	WP_001234626.1	DUF932 domain-containing protein	-
JFX55_RS19630 (4007881)	4007881..4008114	-	234	WP_001119729.1	DUF905 family protein	-
JFX55_RS19635 (4008193)	4008193..4008648	-	456	WP_000581507.1	IrmA family protein	-
JFX55_RS19640 (4008724)	4008724..4010178	-	1455	Protein_3840	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-118)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14307.29 Da
• Isoelectric Point: 7.2922

>T204172 WP_000854902.1 NZ_CP075663:c4005063-4004686 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEF
NACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T204172 NZ_CP100653:c142354-142073 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACACCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13719.50 Da
• Isoelectric Point: 6.6249

>AT204172 WP_001280952.1 NZ_CP075663:c4005484-4005110 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRNQHTVTLYAKGLTCEADTLGSCGYVYLAVYPAQAPATTS

Nucleotide

• Length: 375 bp

>AT204172 NZ_CP100653:c142578-142351 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACATCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Antitoxin

• AlphaFold DB: A0A093DMQ4