Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3488877..3489508 Replicon chromosome
Accession NZ_CP075663
Organism Escherichia coli strain STEC2017-197

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag JFX55_RS17020 Protein ID WP_001259388.1
Coordinates 3488877..3489152 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag JFX55_RS17025 Protein ID WP_000593555.1
Coordinates 3489149..3489508 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX55_RS17005 (3483881) 3483881..3484948 + 1068 WP_000361483.1 HlyD family secretion protein -
JFX55_RS17010 (3484945) 3484945..3487680 + 2736 WP_000149125.1 ribosome-associated ATPase/putative transporter RbbA -
JFX55_RS17015 (3487680) 3487680..3488804 + 1125 WP_001314210.1 ABC transporter permease -
JFX55_RS17020 (3488877) 3488877..3489152 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
JFX55_RS17025 (3489149) 3489149..3489508 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
JFX55_RS17030 (3489628) 3489628..3490029 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
JFX55_RS17035 (3490035) 3490035..3490841 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
JFX55_RS17040 (3490838) 3490838..3491602 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
JFX55_RS17045 (3491602) 3491602..3492435 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
JFX55_RS17050 (3492432) 3492432..3493376 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T204170 WP_001259388.1 NZ_CP075663:3488877-3489152 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T204170 NZ_CP100652:4928410-4928820 [Pseudomonas putida]
ATGCTCAAGTACATGCTCGATACGAATATCTGCATCTTCACGATCAAGAACAAACCCCAGGTAGTTCGTGAAGCGTTCAA
CCGCCACCATGGTCAGCTGGCAATCAGCACCGTCACACTGATGGAGCTTGTTTACGGTGCAGAGAAATCGGCCGCTCCTG
CACGAAATCTCTCGATTGTGGAGGGTTTCGTAGCGCGTCTTGAAGTACTCGACTATGACAGCCATGCAGCAGAACACAGC
GGGCAGCTTCGCTCAGAACTGGCAAAGGCCGGTACGCCCATCGGTCCTTTCGATCAGTTGATTGCCGGGCATGCCCGCGC
GCGGGGGCTTACCTTGGTAACAAACAACCTGCGAGAGTTCATGCGCGTTCCGGGGTTGCGGGTTGAGGATTGGCTGGCCA
TGCCAGGGTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT204170 WP_000593555.1 NZ_CP075663:3489149-3489508 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT204170 NZ_CP100652:4928180-4928410 [Pseudomonas putida]
ATGGAGCAAGGTGCTGTCTTCAAAAGCAATCGTAGCCAGGCCATCCGCTTGCCGAAATCTGTCGCATTTCCCGAAGAGGT
GACCCGCGTGGACATTGTTGCAGTGGGTCGAACCCGCATCATCACTCCCGCTGGTGAATCATGGGATAGCTGGTTTGACG
GCGAAGATGCGAGCCCGGACTTCATGGCCAGCCGCGACCAACCTGCCGACCAGGAACGTGAAGGGTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References