Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2743923..2744420 Replicon chromosome
Accession NZ_CP075663
Organism Escherichia coli strain STEC2017-197

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JFX55_RS13640 Protein ID WP_001173343.1
Coordinates 2743923..2744096 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JFX55_RS13645 Protein ID WP_000398619.1
Coordinates 2744124..2744420 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX55_RS13630 (2740593) 2740593..2742050 + 1458 WP_198790212.1 dipeptide/tripeptide permease DtpC -
JFX55_RS13635 (2742287) 2742287..2743804 + 1518 WP_001295088.1 lysine--tRNA ligase -
JFX55_RS13640 (2743923) 2743923..2744096 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JFX55_RS13645 (2744124) 2744124..2744420 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JFX55_RS13650 (2744646) 2744646..2744918 - 273 WP_000405649.1 GNAT family N-acetyltransferase -
JFX55_RS13655 (2744930) 2744930..2745160 - 231 WP_000371697.1 4Fe-4S mono-cluster protein YjdI -
JFX55_RS13660 (2745341) 2745341..2746972 + 1632 WP_001216487.1 sensor histidine kinase -
JFX55_RS13665 (2746969) 2746969..2747688 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T204168 WP_001173343.1 NZ_CP075663:c2744096-2743923 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T204168 NZ_CP100651:c2270256-2270080 [Bacillus halotolerans]
GTGCTTGGGAAAGTGGGTATCATAGTTGCTTTCCTCATATCTTTAACGGTTCTTACAATCAACAGTCTAACAATAGTTGA
GAAGATAAGAAACCTAAAGAATGGGACAAGCAAAAAGAAAAAGCGTATACGCAAGCGGCTCCGACCAAAGAGACAACGCC
AACGTATACGCCGATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT204168 WP_000398619.1 NZ_CP075663:c2744420-2744124 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT204168 NZ_CP100651:2270197-2270296 [Bacillus halotolerans]
GATTGTAAGAACCGTTAAAGATATGAGGAAAGCAACTATGATACCCACTTTCCCAAGCACTATGTACACCTCCTTTCCTA
TGACTCTATTATAACATAAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References