Detailed information of TA system TA204166

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 2024372..2025209
• Replicon: chromosome
• Accession: NZ_CP075663
• Organism: Escherichia coli strain STEC2017-197

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: JFX55_RS10265
• Protein ID: WP_000227784.1
• Coordinates: 2024667..2025209 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: JFX55_RS10260
• Protein ID: WP_001297137.1
• Coordinates: 2024372..2024683 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX55_RS10235 (2019392)	2019392..2020339	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
JFX55_RS10240 (2020361)	2020361..2022352	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
JFX55_RS10245 (2022342)	2022342..2022956	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
JFX55_RS10250 (2022956)	2022956..2023285	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
JFX55_RS10255 (2023297)	2023297..2024187	+	891	WP_000971336.1	heme o synthase	-
JFX55_RS10260 (2024372)	2024372..2024683	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
JFX55_RS10265 (2024667)	2024667..2025209	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
JFX55_RS10270 (2025265)	2025265..2026200	-	936	WP_001365761.1	tetratricopeptide repeat protein	-
JFX55_RS10275 (2026608)	2026608..2027972	+	1365	WP_001000974.1	MFS transporter	-
JFX55_RS10280 (2028100)	2028100..2028591	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
JFX55_RS10285 (2028759)	2028759..2029670	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T204166 WP_000227784.1 NZ_CP075663:2024667-2025209 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T204166 NZ_CP100651:c2270061-2269810 [Bacillus halotolerans]
ATGAACTTCTCCTTTAGTTCCTACCCATATTATAACATGATCAAGCACATTGTAAACATGAAACGATTCTCGCTTTGGTT
TACCCATATCACATTCATTGGCTTATTCTTGATGTTTCAACTCATTAAAGATTACTTCAGCAGCGAAGCACAAACACTAA
TCAATACAATATTTGTAGTCACATGTATCATTATCTTTCTGATATGGATCATCTATTTTGTATTCCTTAAACTAAGAAAC
AAGTCACACTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT204166 WP_001297137.1 NZ_CP075663:2024372-2024683 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT204166 NZ_CP100651:2270039-2270139 [Bacillus halotolerans]
TAGGAACTAAAGGAGAAGTTCATTCCCCTTTAGCTTAGCTCTCATCGGCGTATACGTTGGCGTTGTCTCTTTGGTCGGAG
CCGCTTGCGTATACGCTTTTT

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3