Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1990295..1990913 Replicon chromosome
Accession NZ_CP075663
Organism Escherichia coli strain STEC2017-197

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JFX55_RS10095 Protein ID WP_001291435.1
Coordinates 1990695..1990913 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JFX55_RS10090 Protein ID WP_000344800.1
Coordinates 1990295..1990669 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX55_RS10080 (1985384) 1985384..1986577 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JFX55_RS10085 (1986600) 1986600..1989749 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
JFX55_RS10090 (1990295) 1990295..1990669 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JFX55_RS10095 (1990695) 1990695..1990913 + 219 WP_001291435.1 HHA domain-containing protein Toxin
JFX55_RS10100 (1991084) 1991084..1991635 + 552 WP_000102564.1 maltose O-acetyltransferase -
JFX55_RS10105 (1991751) 1991751..1992221 + 471 WP_000136192.1 YlaC family protein -
JFX55_RS10110 (1992385) 1992385..1993935 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JFX55_RS10115 (1993977) 1993977..1994330 - 354 WP_000878141.1 DUF1428 family protein -
JFX55_RS10125 (1994709) 1994709..1995020 + 312 WP_000409911.1 MGMT family protein -
JFX55_RS10130 (1995051) 1995051..1995623 - 573 WP_000779833.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T204165 WP_001291435.1 NZ_CP075663:1990695-1990913 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T204165 NZ_CP100651:c1379327-1378581 [Bacillus halotolerans]
ATGCTTTTATTCTTTCAAATCATGGTCTGGTGCACTATGGCCGCTTTAGGCTTATACGTATACGCCACGTGGCGGTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATCCGAAAAACATGGTATTTGCTGTTTGTTTTGGGCGCCATGATATACTGGA
CATATGATCCCTCTTCCCTGTTCACAAACTGGGAACGGTATGTCATTGTCGCTGTCAGCTTTGCTTTAATTGACGCTTTT
ATCTTTTTGAGCGCTTATGTGAAAAAACTGGCCGGCAGTGAACTGGAAACAGACACAAGAGAAATTCTCGAAGAAAACAA
CGAAATGCTCCACATGTATCTCGATCGGCTGAAAACATACCAATATCTATTGAAAAACGAACCGATTCATGTTTATTATG
GAAGTATAGAAGCATATGCTGAAGGAATTGATAAACTGCTGAAAACCTATGCTGATAAAATGAATCTGACGGCCAGCCTT
TGTCACTATTCGACACAGTCTGATAAAGACCGGTTAACCGAGCACATGGAGGATCCCGCTGACGCGCAAACGAGGCTGGA
TCGAAAGGATGTCTATTACGACCAGTATGGAAAAGTAGTGCTCATTCCCTTTACAATTGAGACACAGAGCTATGTCATCA
AACTGACGTCTGACAGCATTGTAACGGAATTTGATTATTTGCTATTCACATCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT204165 WP_000344800.1 NZ_CP075663:1990295-1990669 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT204165 NZ_CP100651:c1378581-1378411 [Bacillus halotolerans]
ATGGAAAACGCGTTTCAAAACGGATGCCATCCGCGGGCAAAGAAACCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCACACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGATAAGTATAAACGAA
AAAAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References