Detailed information of TA system TA204163

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 1102959..1103438
• Replicon: chromosome
• Accession: NZ_CP075663
• Organism: Escherichia coli strain STEC2017-197

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A234Y3J1
• Locus tag: JFX55_RS05670
• Protein ID: WP_001444087.1
• Coordinates: 1103151..1103438 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: A0A234Y267
• Locus tag: JFX55_RS05665
• Protein ID: WP_001090455.1
• Coordinates: 1102959..1103147 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX55_RS05615 (1098032)	1098032..1098208	-	177	WP_000753060.1	hypothetical protein	-
JFX55_RS05620 (1098201)	1098201..1098383	-	183	WP_022581296.1	hypothetical protein	-
JFX55_RS05625 (1098531)	1098531..1098836	-	306	WP_053294093.1	DUF4406 domain-containing protein	-
JFX55_RS05630 (1098833)	1098833..1099114	-	282	WP_072094476.1	DNA-binding protein	-
JFX55_RS05635 (1099147)	1099147..1099908	-	762	WP_180201617.1	DUF1627 domain-containing protein	-
JFX55_RS05640 (1099938)	1099938..1100678	-	741	WP_000790459.1	ATP-binding protein	-
JFX55_RS05645 (1100685)	1100685..1101650	-	966	WP_000054520.1	hypothetical protein	-
JFX55_RS05650 (1101631)	1101631..1102152	-	522	WP_000705386.1	toxin YdaT family protein	-
JFX55_RS05655 (1102136)	1102136..1102414	-	279	WP_000548554.1	YdaS family helix-turn-helix protein	-
JFX55_RS05660 (1102531)	1102531..1102929	+	399	WP_000209117.1	helix-turn-helix domain-containing protein	-
JFX55_RS05665 (1102959)	1102959..1103147	+	189	WP_001090455.1	hypothetical protein	Antitoxin
JFX55_RS05670 (1103151)	1103151..1103438	+	288	WP_001444087.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JFX55_RS05675 (1103710)	1103710..1103862	+	153	WP_000380321.1	DUF1391 family protein	-
JFX55_RS05680 (1103874)	1103874..1104242	+	369	WP_028985920.1	hypothetical protein	-
JFX55_RS05685 (1105027)	1105027..1105215	+	189	WP_000450222.1	cell division inhibition protein DicB	-
JFX55_RS05690 (1105212)	1105212..1105400	+	189	WP_022581374.1	DUF1482 family protein	-
JFX55_RS05695 (1105493)	1105493..1107895	+	2403	WP_215741695.1	exonuclease	-
JFX55_RS05700 (1107957)	1107957..1108226	+	270	WP_000003742.1	excisionase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	stx2B / stx2A	1044563..1116482	71919

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11014.71 Da
• Isoelectric Point: 6.2118

>T204163 WP_001444087.1 NZ_CP075663:1103151-1103438 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVATLSIE
IVSVVHARRQFPKQN

Nucleotide

• Length: 288 bp

>T204163 NZ_CP100647:c4804104-4803952 [Xanthomonas sacchari]
ATGAAGCGACTGACCACCCTGTTGATGCTGACCCTGTTCTGCGCTGGCGTGCTGACCGGCTGCAACACCGTCGCCGGCGC
CGGCAAGGACATGCAGAAGGCCGGCGAGAAGGTGGAAGACACGGCGAAGGATTGCAGCGCGGGCAAGTGCTGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 7249.25 Da
• Isoelectric Point: 8.6832

>AT204163 WP_001090455.1 NZ_CP075663:1102959-1103147 [Escherichia coli]
MNTALSPMVSEFETIEQENSYNEWLRAKVAASLANPRPAIPHDEVERRMAERFAKMRKERSK

Nucleotide

• Length: 189 bp

>AT204163 NZ_CP100647:c4803861-4803727 [Xanthomonas sacchari]
ATGAAGCGTACGTTTGCGTGGATGCTGCTGGCGATGTTCTCGGTGGGCCTGCTGGCCGGTTGCAACACCGTTGCCGGTGC
CGGCAAGGACGTGCAGAAGGCGGGCGAGAAGGTCGAAGACGCCGCCAAGAACTGA

Structures

Toxin

• AlphaFold DB: A0A234Y3J1

Antitoxin

• AlphaFold DB: A0A234Y267