Detailed information of TA system TA204153

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 93794..94316
• Replicon: plasmid pSTEC17_183-b
• Accession: NZ_CP075654
• Organism: Escherichia coli strain STEC17-183

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1PVW2
• Locus tag: JFX43_RS25950
• Protein ID: WP_001681795.1
• Coordinates: 94035..94316 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1Q9V6
• Locus tag: JFX43_RS25945
• Protein ID: WP_001681796.1
• Coordinates: 93794..94045 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX43_RS25920 (JFX43_025920)	89210..89917	-	708	WP_001114349.1	A24 family peptidase	-
JFX43_RS25925 (JFX43_025925)	89877..90359	-	483	WP_021561187.1	lytic transglycosylase domain-containing protein	-
JFX43_RS25930 (JFX43_025930)	90407..90943	-	537	WP_001011500.1	type 4 pilus major pilin	-
JFX43_RS25935 (JFX43_025935)	90993..92093	-	1101	WP_001211427.1	type II secretion system F family protein	-
JFX43_RS25940 (JFX43_025940)	92095..93603	-	1509	WP_012605185.1	ATPase, T2SS/T4P/T4SS family	-
JFX43_RS25945 (JFX43_025945)	93794..94045	+	252	WP_001681796.1	hypothetical protein	Antitoxin
JFX43_RS25950 (JFX43_025950)	94035..94316	+	282	WP_001681795.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JFX43_RS25955 (JFX43_025955)	94355..94573	-	219	WP_175125356.1	hypothetical protein	-
JFX43_RS25960 (JFX43_025960)	94626..95078	-	453	WP_001681794.1	type IV pilus biogenesis protein PilP	-
JFX43_RS25965 (JFX43_025965)	95068..96363	-	1296	WP_016238166.1	type 4b pilus protein PilO2	-
JFX43_RS25970 (JFX43_025970)	96384..98003	-	1620	WP_062859202.1	PilN family type IVB pilus formation outer membrane protein	-
JFX43_RS25975 (JFX43_025975)	98034..98471	-	438	WP_001681791.1	type IV pilus biogenesis protein PilM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..106915	106915

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11122.01 Da
• Isoelectric Point: 10.6249

>T204153 WP_001681795.1 NZ_CP075654:94035-94316 [Escherichia coli]
MTYELEFERRALKEWHKLGHTVREQFKKKLSERLKNPRVPAARLHGHTDRYKIKLRASGYRLVYQVIDEKIVLLVIAVGK
RESSEVYQMADIR

Nucleotide

• Length: 282 bp

>T204153 NZ_CP100601:c3831612-3831295 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9124.33 Da
• Isoelectric Point: 4.0610

>AT204153 WP_001681796.1 NZ_CP075654:93794-94045 [Escherichia coli]
MSYQILTTTAASITELKKNPMGTVAEGEGGAVAILNRNEPAFYCVPPELYAYYRELAEDAELNAIADERMKNPDIVKVSL
DDL

Nucleotide

• Length: 252 bp

>AT204153 NZ_CP100601:c3831302-3831015 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Toxin

• AlphaFold DB: S1PVW2

Antitoxin

• AlphaFold DB: S1Q9V6