Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 34459..34984 Replicon plasmid pSTEC17_183-b
Accession NZ_CP075654
Organism Escherichia coli strain STEC17-183

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag JFX43_RS25620 Protein ID WP_001159871.1
Coordinates 34679..34984 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID B7LIK5
Locus tag JFX43_RS25615 Protein ID WP_001541935.1
Coordinates 34459..34677 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX43_RS25580 (JFX43_025580) 30058..31234 - 1177 Protein_45 IS91 family transposase -
JFX43_RS25585 (JFX43_025585) 31234..31599 - 366 WP_000124098.1 hypothetical protein -
JFX43_RS25590 (JFX43_025590) 31655..32167 + 513 WP_016232721.1 colicin-like pore-forming protein -
JFX43_RS25595 (JFX43_025595) 32185..32532 - 348 WP_016232722.1 colicin E1 family microcin immunity protein -
JFX43_RS25600 (JFX43_025600) 32651..32998 + 348 WP_012605139.1 DUF6404 family protein -
JFX43_RS25605 (JFX43_025605) 33016..33606 - 591 WP_012605140.1 hypothetical protein -
JFX43_RS25610 (JFX43_025610) 33606..33863 - 258 WP_000343099.1 hypothetical protein -
JFX43_RS25615 (JFX43_025615) 34459..34677 + 219 WP_001541935.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
JFX43_RS25620 (JFX43_025620) 34679..34984 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
JFX43_RS25625 (JFX43_025625) 34985..35792 + 808 Protein_54 site-specific integrase -
JFX43_RS25630 (JFX43_025630) 35972..36616 + 645 WP_001144036.1 ParA family protein -
JFX43_RS25635 (JFX43_025635) 36703..37011 + 309 WP_000030199.1 hypothetical protein -
JFX43_RS25640 (JFX43_025640) 37425..38405 + 981 WP_000688514.1 plasmid segregation protein ParM -
JFX43_RS25645 (JFX43_025645) 38398..38814 + 417 WP_001278818.1 plasmid partitioning/stability family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..106915 106915


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T204152 WP_001159871.1 NZ_CP075654:34679-34984 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T204152 NZ_CP100601:c2640805-2639483 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTGGGACGGGATAGCGTAGGGGCGGTGACTCTGCTACCGCCGGG
GGAAGAGGCGCACCTTGAGGGGTTGCGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCGGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
AAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCGCCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8400.40 Da        Isoelectric Point: 4.7405

>AT204152 WP_001541935.1 NZ_CP075654:34459-34677 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMVEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT204152 NZ_CP100601:c2641071-2640805 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A376MGT3

References