Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 18289..18896 Replicon plasmid pSTEC17_183-b
Accession NZ_CP075654
Organism Escherichia coli strain STEC17-183

Toxin (Protein)


Gene name doc Uniprot ID B7LIH8
Locus tag JFX43_RS25495 Protein ID WP_000673985.1
Coordinates 18289..18675 (-) Length 129 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID B7LIH9
Locus tag JFX43_RS25500 Protein ID WP_000493532.1
Coordinates 18672..18896 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX43_RS25470 (JFX43_025470) 13627..14783 + 1157 WP_089538410.1 IS3-like element IS600 family transposase -
JFX43_RS25480 (JFX43_025480) 14834..16498 - 1665 Protein_21 Tn3 family transposase -
JFX43_RS25485 (JFX43_025485) 16659..17249 + 591 WP_012605115.1 recombinase family protein -
JFX43_RS26720 17416..17586 + 171 WP_012605114.1 hypothetical protein -
JFX43_RS25495 (JFX43_025495) 18289..18675 - 387 WP_000673985.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
JFX43_RS25500 (JFX43_025500) 18672..18896 - 225 WP_000493532.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
JFX43_RS25505 (JFX43_025505) 19377..19643 + 267 WP_012605122.1 hypothetical protein -
JFX43_RS25510 (JFX43_025510) 19737..20489 - 753 WP_012605123.1 Gfo/Idh/MocA family oxidoreductase -
JFX43_RS26725 20498..20731 - 234 Protein_28 hypothetical protein -
JFX43_RS25515 (JFX43_025515) 20742..21629 - 888 WP_016232711.1 DMT family transporter -
JFX43_RS25520 (JFX43_025520) 21917..22153 - 237 WP_000887751.1 hypothetical protein -
JFX43_RS26895 22475..22571 + 97 Protein_31 DinQ-like type I toxin DqlB -
JFX43_RS25525 (JFX43_025525) 22638..22817 + 180 WP_000347021.1 hypothetical protein -
JFX43_RS25530 (JFX43_025530) 22933..23307 - 375 Protein_33 recombinase family protein -
JFX43_RS26730 23668..23760 - 93 Protein_34 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..106915 106915
- inside IScluster/Tn - - 8027..17249 9222


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(11-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-52)


Sequences

Toxin        


Download         Length: 129 a.a.        Molecular weight: 14370.62 Da        Isoelectric Point: 7.0732

>T204151 WP_000673985.1 NZ_CP075654:c18675-18289 [Escherichia coli]
MKFVSSQEVIEFHDRLISRDGGVAGMPEPGRADAIIHRVLNMYHYEGVTDIMDLAAVYLVAIARGHIFNDANKRTALFVA
QVFLKRNGVHIISSRISFDEMQIIALNAATGEYNWKMVSDHLKAIILN

Download         Length: 387 bp

>T204151 NZ_CP100601:1916782-1916884 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8361.39 Da        Isoelectric Point: 5.6713

>AT204151 WP_000493532.1 NZ_CP075654:c18896-18672 [Escherichia coli]
MFTCNYSHARDNLASLLELAVDENQPVEIQRRKGGAAVLVDKDAFERYRKAALDAEFAEIMQSHGTAYTELSKR

Download         Length: 225 bp

>AT204151 NZ_CP100601:c1916891-1916746 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A086V9Y9

Antitoxin

Source ID Structure
AlphaFold DB A0A086V9Y8

References