Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 1833..2368 Replicon plasmid pSTEC17_183-b
Accession NZ_CP075654
Organism Escherichia coli strain STEC17-183

Toxin (Protein)


Gene name relE Uniprot ID B7LIF2
Locus tag JFX43_RS25380 Protein ID WP_001536606.1
Coordinates 2081..2368 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0VIP2
Locus tag JFX43_RS25375 Protein ID WP_001132900.1
Coordinates 1833..2084 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX43_RS25370 (JFX43_025370) 1..873 + 873 WP_021553143.1 incFII family plasmid replication initiator RepA -
JFX43_RS25375 (JFX43_025375) 1833..2084 + 252 WP_001132900.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
JFX43_RS25380 (JFX43_025380) 2081..2368 + 288 WP_001536606.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JFX43_RS25385 (JFX43_025385) 2472..3062 + 591 WP_001536605.1 serine acetyltransferase -
JFX43_RS25390 (JFX43_025390) 3165..3818 + 654 WP_016238163.1 ProQ/FINO family protein -
JFX43_RS25395 (JFX43_025395) 3886..4200 + 315 WP_016238162.1 hypothetical protein -
JFX43_RS25400 (JFX43_025400) 4447..4587 - 141 WP_001536601.1 colicin release lysis protein -
JFX43_RS25405 (JFX43_025405) 4685..5014 - 330 WP_000182536.1 colicin transporter -
JFX43_RS25410 (JFX43_025410) 5004..5264 - 261 WP_225374575.1 colicin E5-related ribonuclease -
JFX43_RS25415 (JFX43_025415) 5641..6507 - 867 WP_001536599.1 DMT family transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..106915 106915


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-84)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11086.10 Da        Isoelectric Point: 10.4712

>T204149 WP_001536606.1 NZ_CP075654:2081-2368 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKSIQQQFAKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
REHSNVYNLASERMR

Download         Length: 288 bp

>T204149 NZ_CP100601:731758-732243 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTAATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCGCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9211.41 Da        Isoelectric Point: 4.0780

>AT204149 WP_001132900.1 NZ_CP075654:1833-2084 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Download         Length: 252 bp

>AT204149 NZ_CP100601:731468-731761 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4PMB7

Antitoxin

Source ID Structure
AlphaFold DB V0VIP2

References