Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 38837..39444 Replicon plasmid pSTEC17_183-a
Accession NZ_CP075653
Organism Escherichia coli strain STEC17-183

Toxin (Protein)


Gene name doc Uniprot ID A0A085NWP2
Locus tag JFX43_RS24920 Protein ID WP_000673981.1
Coordinates 39058..39444 (+) Length 129 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID B7LIH9
Locus tag JFX43_RS24915 Protein ID WP_000493532.1
Coordinates 38837..39061 (+) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX43_RS24885 (34096) 34096..35324 + 1229 WP_198800564.1 IS3-like element IS2 family transposase -
JFX43_RS24890 (35633) 35633..37129 + 1497 WP_001040753.1 colicin-like pore-forming protein -
JFX43_RS24895 (37152) 37152..37601 - 450 WP_235155080.1 colicin immunity protein Cui -
JFX43_RS24900 (37737) 37737..38027 - 291 WP_072149383.1 colicin K immunity protein -
JFX43_RS24905 (38051) 38051..38182 + 132 WP_001368419.1 colicin release lysis protein -
JFX43_RS24910 (38416) 38416..38771 - 356 Protein_41 Tn3 family transposase -
JFX43_RS24915 (38837) 38837..39061 + 225 WP_000493532.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
JFX43_RS24920 (39058) 39058..39444 + 387 WP_000673981.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
JFX43_RS24925 (39884) 39884..40342 + 459 Protein_44 IS110 family transposase -
JFX43_RS24930 (40972) 40972..41106 - 135 Protein_45 TieB -
JFX43_RS24935 (41259) 41259..41540 - 282 WP_000780223.1 helix-turn-helix transcriptional regulator -
JFX43_RS24940 (41521) 41521..41850 - 330 WP_000493283.1 type II toxin-antitoxin system RelE/ParE family toxin -
JFX43_RS24945 (42787) 42787..43644 - 858 WP_000131008.1 incFII family plasmid replication initiator RepA -
JFX43_RS24950 (43637) 43637..43711 - 75 WP_001365705.1 RepA leader peptide Tap -
JFX43_RS24955 (43936) 43936..44196 - 261 WP_000083819.1 replication regulatory protein RepA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - hlyD / hlyD / hlyB / hlyB / hlyA / hlyA / hlyA / hlyA / hlyC 1..111930 111930


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-52)


Sequences

Toxin        


Download         Length: 129 a.a.        Molecular weight: 14416.71 Da        Isoelectric Point: 7.0732

>T204143 WP_000673981.1 NZ_CP075653:39058-39444 [Escherichia coli]
MKFVSSQEMIEFHDRLISRDGGVAGMPEPGRADAIIHRVLNMYHYEGVTDIMDLAAVYLVAIARGHIFNDANKRTALFVA
QVFLKRNGIHIISSRISFDEMQIIALNAATGEYNWKMVSDHLKAIILN

Download         Length: 387 bp

>T204143 NZ_CP100596:c2654557-2654414 [Enterococcus faecalis]
ATGAGCGTACTTCCAAAATTTACAGAAAGGAGAGGCCTGTTGTCAGCATATGAAACAATTCAGACGATCCTAGGGTTTGG
TATGTTTACCATTGCTTTGATTGCGCTGATTGTGAAATTGCTTAAAAATGACAATAAAAAATAA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8361.39 Da        Isoelectric Point: 5.6713

>AT204143 WP_000493532.1 NZ_CP075653:38837-39061 [Escherichia coli]
MFTCNYSHARDNLASLLELAVDENQPVEIQRRKGGAAVLVDKDAFERYRKAALDAEFAEIMQSHGTAYTELSKR

Download         Length: 225 bp

>AT204143 NZ_CP100596:2654295-2654481 [Enterococcus faecalis]
TGTGCTATAATGAAAACGAAAAGAGAGATATGCGTCAACATACCTCTCTGATGTAGAGCCGTTTAAGACGGTGACCGATT
TTGTTACAAAAAATAACCGCACTCGATCAAAGTAAACGGTTATTTTTTATTGTCATTTTTAAGCAATTTCACAATCAGCG
CAATCAAAGCAATGGTAAACATACCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A085NWP2

Antitoxin

Source ID Structure
AlphaFold DB A0A086V9Y8

References