Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3663958..3664612 Replicon chromosome
Accession NZ_CP075652
Organism Escherichia coli strain STEC17-183

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag JFX43_RS17805 Protein ID WP_000244777.1
Coordinates 3664205..3664612 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JFX43_RS17800 Protein ID WP_000354046.1
Coordinates 3663958..3664224 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX43_RS17775 (3659127) 3659127..3659870 + 744 WP_001436959.1 SDR family oxidoreductase -
JFX43_RS17780 (3659927) 3659927..3661360 - 1434 WP_001342496.1 6-phospho-beta-glucosidase BglA -
JFX43_RS17785 (3661405) 3661405..3661716 + 312 WP_001182947.1 N(4)-acetylcytidine aminohydrolase -
JFX43_RS17790 (3661880) 3661880..3662539 + 660 WP_000250274.1 hemolysin III family protein -
JFX43_RS17795 (3662735) 3662735..3663715 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
JFX43_RS17800 (3663958) 3663958..3664224 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JFX43_RS17805 (3664205) 3664205..3664612 + 408 WP_000244777.1 protein YgfX Toxin
JFX43_RS17810 (3664652) 3664652..3665173 - 522 WP_001055874.1 flavodoxin FldB -
JFX43_RS17815 (3665285) 3665285..3666181 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JFX43_RS17820 (3666206) 3666206..3666916 + 711 WP_032330662.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JFX43_RS17825 (3666922) 3666922..3668655 + 1734 WP_000813168.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T204131 WP_000244777.1 NZ_CP075652:3664205-3664612 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T204131 NZ_CP100545:c63645-63367 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT204131 WP_000354046.1 NZ_CP075652:3663958-3664224 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT204131 NZ_CP100545:c63914-63645 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCTGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References