Detailed information of TA system TA204130

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3530635..3531467
• Replicon: chromosome
• Accession: NZ_CP075652
• Organism: Escherichia coli strain STEC17-183

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: JFX43_RS17145
• Protein ID: WP_000854816.1
• Coordinates: 3530635..3531009 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: JFX43_RS17150
• Protein ID: WP_001285402.1
• Coordinates: 3531099..3531467 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX43_RS17115 (3526032)	3526032..3527009	+	978	WP_000633239.1	type II secretion system minor pseudopilin GspK	-
JFX43_RS17120 (3527006)	3527006..3528184	+	1179	WP_000094989.1	type II secretion system protein GspL	-
JFX43_RS17125 (3528186)	3528186..3528722	+	537	WP_000942795.1	GspM family type II secretion system protein YghD	-
JFX43_RS17130 (3529003)	3529003..3529845	-	843	WP_001274564.1	DUF4942 domain-containing protein	-
JFX43_RS17135 (3529930)	3529930..3530127	-	198	WP_000772025.1	DUF957 domain-containing protein	-
JFX43_RS17140 (3530147)	3530147..3530638	-	492	WP_000976865.1	DUF5983 family protein	-
JFX43_RS17145 (3530635)	3530635..3531009	-	375	WP_000854816.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
JFX43_RS17150 (3531099)	3531099..3531467	-	369	WP_001285402.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JFX43_RS17155 (3531630)	3531630..3531851	-	222	WP_000692323.1	DUF987 domain-containing protein	-
JFX43_RS17160 (3531920)	3531920..3532396	-	477	WP_001186727.1	RadC family protein	-
JFX43_RS17165 (3532412)	3532412..3532891	-	480	WP_000706981.1	antirestriction protein	-
JFX43_RS17170 (3533154)	3533154..3533972	-	819	WP_001234639.1	DUF932 domain-containing protein	-
JFX43_RS26575 (3534141)	3534141..3534230	-	90	WP_231228162.1	DUF905 family protein	-
JFX43_RS17180 (3534373)	3534373..3534828	-	456	WP_042110819.1	IrmA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14045.05 Da
• Isoelectric Point: 9.1493

>T204130 WP_000854816.1 NZ_CP075652:c3531009-3530635 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLSRLLDQHYGLTLNDTPFSDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPRF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAK

Nucleotide

• Length: 375 bp

>T204130 NZ_CP100544:c4475686-4475375 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13883.62 Da
• Isoelectric Point: 6.3165

>AT204130 WP_001285402.1 NZ_CP075652:c3531467-3531099 [Escherichia coli]
VSDTHHETNYPDDYNNRPWWGLPCTITPCFGARLVQEGNRLYYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELILTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT204130 NZ_CP100544:c4475374-4475084 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG