Detailed information of TA system TA204127

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2724747..2725581
• Replicon: chromosome
• Accession: NZ_CP075652
• Organism: Escherichia coli strain STEC17-183

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: JFX43_RS13280
• Protein ID: WP_001518317.1
• Coordinates: 2724747..2725124 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: JFX43_RS13285
• Protein ID: WP_032330759.1
• Coordinates: 2725213..2725581 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX43_RS13255 (2720115)	2720115..2721038	-	924	WP_000535960.1	carboxylate/amino acid/amine transporter	-
JFX43_RS13260 (2721149)	2721149..2722333	-	1185	WP_001172876.1	sugar efflux transporter	-
JFX43_RS26550 (2722730)	2722730..2722891	-	162	Protein_2594	RhuM family protein	-
JFX43_RS13265 (2723123)	2723123..2723968	-	846	WP_001518322.1	DUF4942 domain-containing protein	-
JFX43_RS13270 (2724053)	2724053..2724250	-	198	WP_000839251.1	DUF957 domain-containing protein	-
JFX43_RS13275 (2724262)	2724262..2724750	-	489	WP_032330753.1	DUF5983 family protein	-
JFX43_RS13280 (2724747)	2724747..2725124	-	378	WP_001518317.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
JFX43_RS13285 (2725213)	2725213..2725581	-	369	WP_032330759.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
JFX43_RS13290 (2725660)	2725660..2725881	-	222	WP_000692312.1	DUF987 domain-containing protein	-
JFX43_RS13295 (2725950)	2725950..2726426	-	477	WP_001518017.1	RadC family protein	-
JFX43_RS13300 (2726442)	2726442..2726915	-	474	WP_032330754.1	antirestriction protein	-
JFX43_RS13305 (2727009)	2727009..2727254	-	246	WP_001518015.1	hypothetical protein	-
JFX43_RS13310 (2727254)	2727254..2728075	-	822	WP_032330755.1	DUF932 domain-containing protein	-
JFX43_RS26555 (2728254)	2728254..2728343	-	90	WP_112031555.1	DUF905 family protein	-
JFX43_RS13320 (2728486)	2728486..2728941	-	456	WP_033804098.1	IrmA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13964.02 Da
• Isoelectric Point: 8.9341

>T204127 WP_001518317.1 NZ_CP075652:c2725124-2724747 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKYPEAKR

Nucleotide

• Length: 378 bp

>T204127 NZ_CP100544:4004413-4004754 [Escherichia coli]
ATGACTGACACGCATTTTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGCCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGCGGTAGATGTCAAAGTAATGGAAGGCTGTACTGTCCTCACCGCCCAACAACCCGCCGCCGAAGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGATTTTATTGGGGTGATTGC
CGGTAAACAGAAAGTCGCCTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13603.35 Da
• Isoelectric Point: 5.8746

>AT204127 WP_032330759.1 NZ_CP075652:c2725581-2725213 [Escherichia coli]
VSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT204127 NZ_CP100544:c4004418-4004342 [Escherichia coli]
AGTCATAACTGCTATTCCTTGAAAATAGTGATTGTGATGAGTGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT