Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 2088174..2088769 Replicon chromosome
Accession NZ_CP075652
Organism Escherichia coli strain STEC17-183

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag JFX43_RS10265 Protein ID WP_000239579.1
Coordinates 2088174..2088524 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag JFX43_RS10270 Protein ID WP_001223208.1
Coordinates 2088518..2088769 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX43_RS10245 (2083620) 2083620..2084642 - 1023 WP_001368084.1 ABC transporter permease -
JFX43_RS10250 (2084656) 2084656..2086158 - 1503 WP_000210554.1 sugar ABC transporter ATP-binding protein -
JFX43_RS10255 (2086298) 2086298..2087254 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
JFX43_RS10260 (2087564) 2087564..2088094 + 531 WP_000055075.1 inorganic diphosphatase -
JFX43_RS10265 (2088174) 2088174..2088524 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
JFX43_RS10270 (2088518) 2088518..2088769 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
JFX43_RS10275 (2088981) 2088981..2089322 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
JFX43_RS10280 (2089325) 2089325..2093104 - 3780 WP_032330859.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T204124 WP_000239579.1 NZ_CP075652:c2088524-2088174 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T204124 NZ_CP100544:3424564-3424782 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT204124 WP_001223208.1 NZ_CP075652:c2088769-2088518 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT204124 NZ_CP100544:3424164-3424538 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References