Detailed information of TA system TA204120

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1448900..1449737
• Replicon: chromosome
• Accession: NZ_CP075652
• Organism: Escherichia coli strain STEC17-183

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: JFX43_RS07335
• Protein ID: WP_000227784.1
• Coordinates: 1449195..1449737 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: JFX43_RS07330
• Protein ID: WP_001297137.1
• Coordinates: 1448900..1449211 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX43_RS07305 (1443920)	1443920..1444867	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
JFX43_RS07310 (1444889)	1444889..1446880	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
JFX43_RS07315 (1446870)	1446870..1447484	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
JFX43_RS07320 (1447484)	1447484..1447813	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
JFX43_RS07325 (1447825)	1447825..1448715	+	891	WP_000971336.1	heme o synthase	-
JFX43_RS07330 (1448900)	1448900..1449211	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
JFX43_RS07335 (1449195)	1449195..1449737	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
JFX43_RS07340 (1449793)	1449793..1450728	-	936	WP_001368479.1	tetratricopeptide repeat protein	-
JFX43_RS07345 (1451136)	1451136..1452500	+	1365	WP_001000974.1	MFS transporter	-
JFX43_RS07350 (1452628)	1452628..1453119	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
JFX43_RS07355 (1453287)	1453287..1454198	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T204120 WP_000227784.1 NZ_CP075652:1449195-1449737 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T204120 NZ_CP100544:1799847-1800101 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT204120 WP_001297137.1 NZ_CP075652:1448900-1449211 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT204120 NZ_CP100544:1799599-1799850 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3