Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1414821..1415439 Replicon chromosome
Accession NZ_CP075652
Organism Escherichia coli strain STEC17-183

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JFX43_RS07165 Protein ID WP_001291435.1
Coordinates 1415221..1415439 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JFX43_RS07160 Protein ID WP_000344800.1
Coordinates 1414821..1415195 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX43_RS07150 (1409910) 1409910..1411103 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JFX43_RS07155 (1411126) 1411126..1414275 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
JFX43_RS07160 (1414821) 1414821..1415195 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JFX43_RS07165 (1415221) 1415221..1415439 + 219 WP_001291435.1 HHA domain-containing protein Toxin
JFX43_RS07170 (1415611) 1415611..1416162 + 552 WP_151042689.1 maltose O-acetyltransferase -
JFX43_RS07175 (1416278) 1416278..1416748 + 471 WP_000136192.1 YlaC family protein -
JFX43_RS07180 (1416912) 1416912..1418462 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JFX43_RS07185 (1418504) 1418504..1418857 - 354 WP_000878141.1 DUF1428 family protein -
JFX43_RS07195 (1419236) 1419236..1419547 + 312 WP_000409911.1 MGMT family protein -
JFX43_RS07200 (1419578) 1419578..1420150 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T204119 WP_001291435.1 NZ_CP075652:1415221-1415439 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T204119 NZ_CP100544:1799599-1799850 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT204119 WP_000344800.1 NZ_CP075652:1414821-1415195 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT204119 NZ_CP100544:1799847-1800101 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References