Detailed information of TA system TA204118

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 229751..230389
• Replicon: chromosome
• Accession: NZ_CP075652
• Organism: Escherichia coli strain STEC17-183

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: JFX43_RS01145
• Protein ID: WP_000813794.1
• Coordinates: 230213..230389 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JFX43_RS01140
• Protein ID: WP_001270286.1
• Coordinates: 229751..230167 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX43_RS01120 (224903)	224903..225844	-	942	WP_001251315.1	ABC transporter permease	-
JFX43_RS01125 (225845)	225845..226858	-	1014	WP_198800525.1	ABC transporter ATP-binding protein	-
JFX43_RS01130 (226876)	226876..228021	-	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
JFX43_RS01135 (228266)	228266..229672	-	1407	WP_032331455.1	PLP-dependent aminotransferase family protein	-
JFX43_RS01140 (229751)	229751..230167	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
JFX43_RS01145 (230213)	230213..230389	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
JFX43_RS01150 (230611)	230611..230841	+	231	WP_000494244.1	YncJ family protein	-
JFX43_RS01155 (230933)	230933..232894	-	1962	WP_024178299.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
JFX43_RS01160 (232967)	232967..233503	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
JFX43_RS01165 (233595)	233595..234770	+	1176	WP_001236258.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	234810..236075	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T204118 WP_000813794.1 NZ_CP075652:c230389-230213 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T204118 NZ_CP100544:1284123-1284521 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT204118 WP_001270286.1 NZ_CP075652:c230167-229751 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT204118 NZ_CP100544:1283896-1284123 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Toxin

• PDB: 6HPB