Detailed information of TA system TA204116

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 49263..49742
• Replicon: chromosome
• Accession: NZ_CP075652
• Organism: Escherichia coli strain STEC17-183

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A3Y4Y761
• Locus tag: JFX43_RS00240
• Protein ID: WP_032151266.1
• Coordinates: 49263..49550 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: A0A3Y4Y793
• Locus tag: JFX43_RS00245
• Protein ID: WP_021577224.1
• Coordinates: 49557..49742 (-)
• Length: 62 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX43_RS00215 (45081)	45081..45332	-	252	WP_000005551.1	excisionase family protein	-
JFX43_RS00220 (45404)	45404..47875	-	2472	WP_021565031.1	exonuclease	-
JFX43_RS00225 (47971)	47971..48159	-	189	WP_000199480.1	DUF1482 family protein	-
JFX43_RS00230 (48156)	48156..48344	-	189	WP_000449172.1	cell division inhibition protein DicB	-
JFX43_RS00235 (48828)	48828..48980	-	153	WP_032151319.1	DUF1391 family protein	-
JFX43_RS00240 (49263)	49263..49550	-	288	WP_032151266.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JFX43_RS00245 (49557)	49557..49742	-	186	WP_021577224.1	hypothetical protein	Antitoxin
JFX43_RS00250 (49763)	49763..50134	-	372	WP_021577225.1	hypothetical protein	-
JFX43_RS00255 (50237)	50237..50518	+	282	WP_021577226.1	YdaS family helix-turn-helix protein	-
JFX43_RS00260 (50522)	50522..50959	+	438	WP_021577227.1	toxin YdaT family protein	-
JFX43_RS00265 (51046)	51046..52056	+	1011	WP_198800398.1	DUF1376 domain-containing protein	-
JFX43_RS00270 (52049)	52049..52510	+	462	WP_000139447.1	replication protein P	-
JFX43_RS00275 (52544)	52544..53269	+	726	WP_000450636.1	DUF1627 domain-containing protein	-
JFX43_RS00280 (53285)	53285..53677	+	393	WP_016237929.1	DUF977 family protein	-
JFX43_RS00290 (53674)	53674..54024	+	351	WP_072006479.1	DUF4406 domain-containing protein	-
JFX43_RS00295 (54021)	54021..54245	+	225	WP_021577603.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	46..116700	116654

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10959.63 Da
• Isoelectric Point: 6.2092

>T204116 WP_032151266.1 NZ_CP075652:c49550-49263 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDINAAERLWQRLRACVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSGIE
IVNVVHARRQFPITS

Nucleotide

• Length: 288 bp

>T204116 NZ_CP100544:848071-848478 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 62 a.a.
• Molecular weight: 7190.13 Da
• Isoelectric Point: 5.1069

>AT204116 WP_021577224.1 NZ_CP075652:c49742-49557 [Escherichia coli]
MNRALSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDEVERRMAERFAEMRKERI

Nucleotide

• Length: 186 bp

>AT204116 NZ_CP100544:847824-848090 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A3Y4Y761

Antitoxin

• AlphaFold DB: A0A3Y4Y793