Detailed information of TA system TA204113

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4605248..4606080
• Replicon: chromosome
• Accession: NZ_CP075649
• Organism: Escherichia coli strain STEC17-190

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: JFX56_RS22100
• Protein ID: WP_000854816.1
• Coordinates: 4605248..4605622 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: JFX56_RS22105
• Protein ID: WP_001285402.1
• Coordinates: 4605712..4606080 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX56_RS22065 (4601055)	4601055..4601331	-	277	Protein_4347	phosphoethanolamine transferase	-
JFX56_RS22070 (4601417)	4601417..4603030	-	1614	WP_001499133.1	IS66 family transposase	-
JFX56_RS22075 (4603061)	4603061..4603411	-	351	WP_000624684.1	IS66 family insertion sequence element accessory protein TnpB	-
JFX56_RS22080 (4603408)	4603408..4603833	-	426	WP_000422722.1	transposase	-
JFX56_RS22085 (4603918)	4603918..4604466	-	549	Protein_4351	DUF4942 domain-containing protein	-
JFX56_RS22090 (4604551)	4604551..4604748	-	198	WP_032209866.1	DUF957 domain-containing protein	-
JFX56_RS22095 (4604760)	4604760..4605251	-	492	WP_000976864.1	DUF5983 family protein	-
JFX56_RS22100 (4605248)	4605248..4605622	-	375	WP_000854816.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
JFX56_RS22105 (4605712)	4605712..4606080	-	369	WP_001285402.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JFX56_RS22110 (4606243)	4606243..4606464	-	222	WP_000692323.1	DUF987 domain-containing protein	-
JFX56_RS22115 (4606533)	4606533..4607009	-	477	WP_136755805.1	RadC family protein	-
JFX56_RS22120 (4607025)	4607025..4607504	-	480	WP_000706981.1	antirestriction protein	-
JFX56_RS22125 (4607767)	4607767..4608585	-	819	WP_001234638.1	DUF932 domain-containing protein	-
JFX56_RS22130 (4608754)	4608754..4608966	-	213	WP_000941284.1	DUF905 family protein	-
JFX56_RS22135 (4608984)	4608984..4609439	-	456	WP_024226900.1	IrmA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14045.05 Da
• Isoelectric Point: 9.1493

>T204113 WP_000854816.1 NZ_CP075649:c4605622-4605248 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLSRLLDQHYGLTLNDTPFSDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPRF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAK

Nucleotide

• Length: 375 bp

>T204113 NZ_CP100544:264226-264456 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13883.62 Da
• Isoelectric Point: 6.3165

>AT204113 WP_001285402.1 NZ_CP075649:c4606080-4605712 [Escherichia coli]
VSDTHHETNYPDDYNNRPWWGLPCTITPCFGARLVQEGNRLYYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELILTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT204113 NZ_CP100544:264453-264812 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA