Detailed information of TA system TA204110

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4400607..4402290
• Replicon: chromosome
• Accession: NZ_CP075649
• Organism: Escherichia coli strain STEC17-190

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: -
• Locus tag: JFX56_RS21035
• Protein ID: WP_000361918.1
• Coordinates: 4401373..4402290 (+)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: I2UPX5
• Locus tag: JFX56_RS21030
• Protein ID: WP_000157137.1
• Coordinates: 4400607..4401380 (+)
• Length: 258 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX56_RS21015 (4396475)	4396475..4398559	+	2085	WP_001462982.1	protease Lon-related BREX system protein BrxL	-
JFX56_RS21020 (4398678)	4398678..4400027	+	1350	WP_000766444.1	phospholipase D-like domain-containing protein	-
JFX56_RS21025 (4400203)	4400203..4400523	-	321	Protein_4142	IS3 family transposase	-
JFX56_RS21030 (4400607)	4400607..4401380	+	774	WP_000157137.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
JFX56_RS21035 (4401373)	4401373..4402290	+	918	WP_000361918.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
JFX56_RS21040 (4402804)	4402804..4403466	-	663	WP_000357928.1	hypothetical protein	-
JFX56_RS21045 (4403539)	4403539..4404180	-	642	Protein_4146	integrase arm-type DNA-binding domain-containing protein	-
JFX56_RS21050 (4404647)	4404647..4404790	-	144	Protein_4147	hypothetical protein	-
JFX56_RS21055 (4404875)	4404875..4405072	-	198	WP_001327226.1	DUF957 domain-containing protein	-
JFX56_RS21060 (4405092)	4405092..4405580	-	489	WP_000777664.1	DUF5983 family protein	-
JFX56_RS21065 (4405577)	4405577..4405954	-	378	WP_000854919.1	TA system toxin CbtA family protein	-
JFX56_RS21070 (4406001)	4406001..4406375	-	375	WP_063114613.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	-
JFX56_RS21075 (4406455)	4406455..4406676	-	222	WP_000692350.1	DUF987 domain-containing protein	-
JFX56_RS21080 (4406763)	4406763..4407239	-	477	WP_001186725.1	RadC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	papH / papC / papD	4400607..4460037	59430

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (13-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34962.10 Da
• Isoelectric Point: 4.9206

>T204110 WP_000361918.1 NZ_CP075649:4401373-4402290 [Escherichia coli]
MDKTSPYYRQVALLVRALPWVANESCFALKGGTAINLFIHDFPRLSVDIDLAWIPLESREVALPNVREALTRIAAELKAQ
TGIRAVLQTHNPDEMRIIVTTDDAQIKIEVSPVARGTLYPPQERDIVGAVEEEFGFAALPVVSLPDLYGGKLCAALDRQH
PRDFYDVKMLLDTQGIDRSIFNGFIVYLLSHNRPLSEVLNPRWKDITEPFQNEFSGMTFEEVSLEELKEVPERMLSTLKT
HFTQQDFDFLMSFKRGEPDWNLAPEPHIQNLPAVQWKLRNIGQMSEEKRAQSLEKLEKVMTEWLR

Nucleotide

• Length: 918 bp

>T204110 NZ_CP100542:c5114137-5113754 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGTACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 258 a.a.
• Molecular weight: 29333.76 Da
• Isoelectric Point: 9.9889

>AT204110 WP_000157137.1 NZ_CP075649:4400607-4401380 [Escherichia coli]
MASKLNWLLQNTSPGSLVLQSWLTRNNISPSLAFKYAQNGWLIKLRAGVYARAGREPDWSDALWCLQNQLEAPVHLAGLS
SLSWQGRSHYLQLKQNQCWLSVENKAILPKWFKEFPAVEWLMVSALKLPDLDEKYRTELEVKGKRITASTPELAAYELLS
AVPQEISFEHAAELFQGLVNLNPRKVEKLLTLSQSVQTKRLYLFFASFYEHAWFKRIDTSKINLGSGNRQIVVNGKLNTQ
YQITVPENFTKKGISHG

Nucleotide

• Length: 774 bp

>AT204110 NZ_CP100542:c5114379-5114137 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A0H0N3W2