Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1477383..1478008 Replicon chromosome
Accession NZ_CP075649
Organism Escherichia coli strain STEC17-190

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag JFX56_RS06980 Protein ID WP_000911329.1
Coordinates 1477610..1478008 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag JFX56_RS06975 Protein ID WP_000450524.1
Coordinates 1477383..1477610 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX56_RS06950 (1473185) 1473185..1473655 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
JFX56_RS06955 (1473655) 1473655..1474227 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
JFX56_RS06960 (1474373) 1474373..1475251 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
JFX56_RS06965 (1475268) 1475268..1476302 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
JFX56_RS06970 (1476515) 1476515..1477228 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
JFX56_RS06975 (1477383) 1477383..1477610 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
JFX56_RS06980 (1477610) 1477610..1478008 + 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
JFX56_RS06985 (1478155) 1478155..1479018 + 864 WP_001267498.1 neutral zinc metallopeptidase -
JFX56_RS06990 (1479033) 1479033..1481048 + 2016 WP_000829393.1 tRNA cytosine(34) acetyltransferase TmcA -
JFX56_RS06995 (1481122) 1481122..1481820 + 699 WP_000679812.1 esterase -
JFX56_RS07000 (1481930) 1481930..1482130 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T204091 WP_000911329.1 NZ_CP075649:1477610-1478008 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T204091 NZ_CP100530:c3847530-3847132 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT204091 WP_000450524.1 NZ_CP075649:1477383-1477610 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT204091 NZ_CP100530:c3847826-3847533 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References