Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1006796..1007450 Replicon chromosome
Accession NZ_CP075649
Organism Escherichia coli strain STEC17-190

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag JFX56_RS04830 Protein ID WP_000244772.1
Coordinates 1007043..1007450 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JFX56_RS04825 Protein ID WP_000354046.1
Coordinates 1006796..1007062 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX56_RS04800 (1002084) 1002084..1002827 + 744 WP_001436959.1 SDR family oxidoreductase -
JFX56_RS04805 (1002884) 1002884..1004317 - 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
JFX56_RS04810 (1004362) 1004362..1004673 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
JFX56_RS04815 (1004837) 1004837..1005496 + 660 WP_000250274.1 hemolysin III family protein -
JFX56_RS04820 (1005573) 1005573..1006553 - 981 WP_198779771.1 tRNA-modifying protein YgfZ -
JFX56_RS04825 (1006796) 1006796..1007062 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JFX56_RS04830 (1007043) 1007043..1007450 + 408 WP_000244772.1 protein YgfX Toxin
JFX56_RS04835 (1007490) 1007490..1008011 - 522 WP_001055874.1 flavodoxin FldB -
JFX56_RS04840 (1008123) 1008123..1009019 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JFX56_RS04845 (1009044) 1009044..1009754 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JFX56_RS04850 (1009760) 1009760..1011493 + 1734 WP_000813213.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T204089 WP_000244772.1 NZ_CP075649:1007043-1007450 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T204089 NZ_CP100530:3640729-3640947 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT204089 WP_000354046.1 NZ_CP075649:1006796-1007062 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT204089 NZ_CP100530:3640329-3640703 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References