Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 350820..351620 Replicon chromosome
Accession NZ_CP075649
Organism Escherichia coli strain STEC17-190

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag JFX56_RS01620 Protein ID WP_000342449.1
Coordinates 351093..351620 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag JFX56_RS01615 Protein ID WP_001277108.1
Coordinates 350820..351086 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX56_RS01595 (346478) 346478..347146 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
JFX56_RS01600 (347139) 347139..348197 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
JFX56_RS01605 (348442) 348442..349296 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
JFX56_RS01610 (349567) 349567..350670 + 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
JFX56_RS01615 (350820) 350820..351086 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
JFX56_RS01620 (351093) 351093..351620 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
JFX56_RS01625 (351617) 351617..352000 - 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
JFX56_RS01630 (352424) 352424..353533 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
JFX56_RS01635 (353581) 353581..354507 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
JFX56_RS01640 (354504) 354504..355781 + 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
JFX56_RS01645 (355778) 355778..356545 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T204086 WP_000342449.1 NZ_CP075649:351093-351620 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T204086 NZ_CP100530:2813980-2814087 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT204086 WP_001277108.1 NZ_CP075649:350820-351086 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT204086 NZ_CP100530:c2813933-2813867 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References