Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 323747..324378 Replicon chromosome
Accession NZ_CP075649
Organism Escherichia coli strain STEC17-190

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag JFX56_RS01460 Protein ID WP_001259385.1
Coordinates 323747..324022 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q31VE4
Locus tag JFX56_RS01465 Protein ID WP_000593554.1
Coordinates 324019..324378 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX56_RS01445 (318751) 318751..319818 + 1068 WP_000361467.1 HlyD family secretion protein -
JFX56_RS01450 (319815) 319815..322550 + 2736 WP_198779769.1 ribosome-associated ATPase/putative transporter RbbA -
JFX56_RS01455 (322550) 322550..323674 + 1125 WP_001314210.1 ABC transporter permease -
JFX56_RS01460 (323747) 323747..324022 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
JFX56_RS01465 (324019) 324019..324378 + 360 WP_000593554.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
JFX56_RS01470 (324428) 324428..325288 - 861 WP_001131783.1 class II aldolase -
JFX56_RS01475 (325320) 325320..325589 - 270 WP_000933060.1 HPr family phosphocarrier protein -
JFX56_RS01480 (325579) 325579..327087 - 1509 WP_001298718.1 FGGY-family carbohydrate kinase -
JFX56_RS01485 (327080) 327080..328438 - 1359 WP_001298729.1 PTS galactitol transporter subunit IIC -
JFX56_RS01490 (328515) 328515..328796 - 282 WP_000084022.1 PTS sugar transporter subunit IIB -
JFX56_RS01495 (328793) 328793..329266 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 316130..330637 14507


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T204085 WP_001259385.1 NZ_CP075649:323747-324022 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T204085 NZ_CP100530:c2537632-2537456 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13359.25 Da        Isoelectric Point: 4.8838

>AT204085 WP_000593554.1 NZ_CP075649:324019-324378 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT204085 NZ_CP100530:c2537410-2536994 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A6H2GM94

References