Detailed information of TA system TA204079

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 5119..5701
• Replicon: plasmid pS19_141-b
• Accession: NZ_CP075642
• Organism: Escherichia coli strain S19-141

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A403LBG1
• Locus tag: JFX59_RS25195
• Protein ID: WP_042968292.1
• Coordinates: 5119..5415 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: JFX59_RS25200
• Protein ID: WP_047082007.1
• Coordinates: 5417..5701 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX59_RS25940	2105..2257	+	153	Protein_1	DUF5431 family protein	-
JFX59_RS25170 (JFX59_025170)	2202..2324	+	123	WP_223202818.1	Hok/Gef family protein	-
JFX59_RS25175 (JFX59_025175)	2474..3070	+	597	WP_000125165.1	ProQ/FINO family protein	-
JFX59_RS25180 (JFX59_025180)	3088..3372	-	285	WP_001542021.1	hypothetical protein	-
JFX59_RS25185 (JFX59_025185)	3451..4116	-	666	WP_000532148.1	division plane positioning ATPase MipZ	-
JFX59_RS25190 (JFX59_025190)	4620..4949	+	330	WP_047082006.1	hypothetical protein	-
JFX59_RS25195 (JFX59_025195)	5119..5415	+	297	WP_042968292.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JFX59_RS25200 (JFX59_025200)	5417..5701	+	285	WP_047082007.1	putative addiction module antidote protein	Antitoxin
JFX59_RS25205 (JFX59_025205)	5954..6208	+	255	WP_086217910.1	hypothetical protein	-
JFX59_RS25210 (JFX59_025210)	6205..6441	+	237	WP_047082009.1	transcriptional regulator	-
JFX59_RS25215 (JFX59_025215)	6453..6599	+	147	WP_155953439.1	hypothetical protein	-
JFX59_RS25220 (JFX59_025220)	7153..7479	+	327	WP_032716095.1	hypothetical protein	-
JFX59_RS25225 (JFX59_025225)	7520..7729	+	210	WP_032716096.1	hypothetical protein	-
JFX59_RS25230 (JFX59_025230)	7827..8051	+	225	WP_000814383.1	hypothetical protein	-
JFX59_RS25235 (JFX59_025235)	8103..8360	+	258	WP_042968288.1	hypothetical protein	-
JFX59_RS25240 (JFX59_025240)	8354..8605	+	252	WP_022645142.1	transcriptional regulator	-
JFX59_RS25245 (JFX59_025245)	9143..9448	+	306	WP_072096363.1	hypothetical protein	-
JFX59_RS25250 (JFX59_025250)	9754..10041	+	288	WP_000606467.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..42892	42892

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (46-90)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11283.29 Da
• Isoelectric Point: 10.1814

>T204079 WP_042968292.1 NZ_CP075642:5119-5415 [Escherichia coli]
MIEMKQTPEFEKWLKSLKDKIAKAKILMRLERMEEGNFGDVEPVGDGLSELRIHQGKGYRIYFGNKNNQIVILLCGGDKS
TQQLDIKKAKILAKKWGF

Nucleotide

• Length: 297 bp

>T204079 NZ_CP100530:1079048-1079383 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10307.68 Da
• Isoelectric Point: 6.3398

>AT204079 WP_047082007.1 NZ_CP075642:5417-5701 [Escherichia coli]
MNGGLKKFDVVEYLEDEEDIQAYLNAAIEEGDTKYFFKALGNIARARNISQLSKDAGMSREGIYKALSGEGNPSFNTVAR
ITKALGLKLQFKGA

Nucleotide

• Length: 285 bp

>AT204079 NZ_CP100530:1078800-1079048 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A403LBG1