Detailed information of TA system TA204078

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 127907..128432
• Replicon: plasmid pS19_141-a
• Accession: NZ_CP075641
• Organism: Escherichia coli strain S19-141

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1PFV8
• Locus tag: JFX59_RS25120
• Protein ID: WP_001159871.1
• Coordinates: 127907..128212 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: JFX59_RS25125
• Protein ID: WP_198779936.1
• Coordinates: 128214..128432 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX59_RS25090 (123174)	123174..124444	+	1271	Protein_131	translesion error-prone DNA polymerase V subunit UmuC	-
JFX59_RS25095 (124449)	124449..124841	-	393	WP_000340836.1	plasmid partitioning/stability family protein	-
JFX59_RS25100 (124846)	124846..125817	-	972	WP_001103695.1	plasmid segregation protein ParM	-
JFX59_RS25105 (126046)	126046..126690	+	645	WP_000633912.1	division plane positioning ATPase MipZ	-
JFX59_RS25110 (126684)	126684..126959	+	276	WP_000239527.1	hypothetical protein	-
JFX59_RS25115 (127097)	127097..127906	-	810	WP_000016958.1	site-specific integrase	-
JFX59_RS25120 (127907)	127907..128212	-	306	WP_001159871.1	type II toxin-antitoxin system toxin CcdB	Toxin
JFX59_RS25125 (128214)	128214..128432	-	219	WP_198779936.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
JFX59_RS25130 (129028)	129028..129288	+	261	WP_000343084.1	hypothetical protein	-
JFX59_RS25135 (129285)	129285..129875	+	591	WP_000194564.1	hypothetical protein	-
JFX59_RS25140 (129893)	129893..130240	-	348	WP_000142428.1	DUF6404 family protein	-
JFX59_RS25145 (130494)	130494..130703	+	210	WP_225303885.1	colicin E1 immunity protein	-
JFX59_RS25150 (130710)	130710..131030	-	321	WP_001365556.1	colicin-like pore-forming protein	-
JFX59_RS25155 (131140)	131140..131871	-	732	WP_000850413.1	conjugal transfer complement resistance protein TraT	-
JFX59_RS25160 (132148)	132148..132300	-	153	Protein_145	IS481 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyA / hlyC / espP	1..133041	133041

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11692.49 Da
• Isoelectric Point: 6.4674

>T204078 WP_001159871.1 NZ_CP075641:c128212-127907 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T204078 NZ_CP100530:953489-953896 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTAGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8357.33 Da
• Isoelectric Point: 4.5203

>AT204078 WP_198779936.1 NZ_CP075641:c128432-128214 [Escherichia coli]
MKLRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT204078 NZ_CP100530:953242-953508 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A829CCE8