Detailed information of TA system TA204068

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 3542943..3543529
• Replicon: chromosome
• Accession: NZ_CP075640
• Organism: Escherichia coli strain S19-141

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0H0RQZ4
• Locus tag: JFX59_RS16985
• Protein ID: WP_042014162.1
• Coordinates: 3542943..3543173 (+)
• Length: 77 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JFX59_RS16990
• Protein ID: WP_000593552.1
• Coordinates: 3543170..3543529 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX59_RS16975 (3538980)	3538980..3541715	+	2736	WP_024213463.1	ribosome-associated ATPase/putative transporter RbbA	-
JFX59_RS16980 (3541715)	3541715..3542839	+	1125	WP_001216257.1	ABC transporter permease	-
JFX59_RS16985 (3542943)	3542943..3543173	+	231	WP_042014162.1	type II toxin-antitoxin system HicA family toxin	Toxin
JFX59_RS16990 (3543170)	3543170..3543529	+	360	WP_000593552.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
JFX59_RS16995 (3543649)	3543649..3544050	-	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
JFX59_RS17000 (3544056)	3544056..3544862	-	807	WP_024213464.1	nickel import ATP-binding protein NikE	-
JFX59_RS17005 (3544859)	3544859..3545623	-	765	WP_001136236.1	nickel import ATP-binding protein NikD	-
JFX59_RS17010 (3545623)	3545623..3546456	-	834	WP_001008963.1	nickel ABC transporter permease subunit NikC	-
JFX59_RS17015 (3546453)	3546453..3547397	-	945	WP_000947068.1	nickel ABC transporter permease subunit NikB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (15-65)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 77 a.a.
• Molecular weight: 8419.79 Da
• Isoelectric Point: 8.5518

>T204068 WP_042014162.1 NZ_CP075640:3542943-3543173 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Nucleotide

• Length: 231 bp

>T204068 NZ_CP100525:c4084854-4084504 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCAGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGAAATAA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13462.37 Da
• Isoelectric Point: 5.1987

>AT204068 WP_000593552.1 NZ_CP075640:3543170-3543529 [Escherichia coli]
MIKLKTPNFMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT204068 NZ_CP100525:c4085099-4084848 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCTACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A0H0RQZ4