Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1820536..1821373 Replicon chromosome
Accession NZ_CP075640
Organism Escherichia coli strain S19-141

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag JFX59_RS08975 Protein ID WP_000227784.1
Coordinates 1820831..1821373 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag JFX59_RS08970 Protein ID WP_001297137.1
Coordinates 1820536..1820847 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX59_RS08945 (1815556) 1815556..1816503 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
JFX59_RS08950 (1816525) 1816525..1818516 + 1992 WP_038347496.1 cytochrome o ubiquinol oxidase subunit I -
JFX59_RS08955 (1818506) 1818506..1819120 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
JFX59_RS08960 (1819120) 1819120..1819449 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
JFX59_RS08965 (1819461) 1819461..1820351 + 891 WP_000971336.1 heme o synthase -
JFX59_RS08970 (1820536) 1820536..1820847 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
JFX59_RS08975 (1820831) 1820831..1821373 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
JFX59_RS08980 (1821429) 1821429..1822364 - 936 WP_001368479.1 tetratricopeptide repeat protein -
JFX59_RS08985 (1822772) 1822772..1824136 + 1365 WP_024213548.1 MFS transporter -
JFX59_RS08990 (1824264) 1824264..1824755 - 492 WP_001138904.1 nucleotide binding protein YajQ -
JFX59_RS08995 (1824923) 1824923..1825834 + 912 WP_000705874.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T204061 WP_000227784.1 NZ_CP075640:1820831-1821373 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T204061 NZ_CP100525:c2374392-2374216 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT204061 WP_001297137.1 NZ_CP075640:1820536-1820847 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT204061 NZ_CP100525:c2374170-2373754 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References