Detailed information of TA system TA204059

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 707505..708143
• Replicon: chromosome
• Accession: NZ_CP075640
• Organism: Escherichia coli strain S19-141

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: JFX59_RS03575
• Protein ID: WP_000813794.1
• Coordinates: 707967..708143 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JFX59_RS03570
• Protein ID: WP_077637634.1
• Coordinates: 707505..707921 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX59_RS03550 (702658)	702658..703598	-	941	Protein_702	ABC transporter permease	-
JFX59_RS03555 (703599)	703599..704612	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
JFX59_RS03560 (704630)	704630..705775	-	1146	WP_024213730.1	ABC transporter substrate-binding protein	-
JFX59_RS03565 (706020)	706020..707426	-	1407	WP_000760592.1	PLP-dependent aminotransferase family protein	-
JFX59_RS03570 (707505)	707505..707921	-	417	WP_077637634.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
JFX59_RS03575 (707967)	707967..708143	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
JFX59_RS03580 (708365)	708365..708595	+	231	WP_000494244.1	YncJ family protein	-
JFX59_RS03585 (708687)	708687..710648	-	1962	WP_024178299.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
JFX59_RS03590 (710721)	710721..711257	-	537	WP_024213732.1	DNA-binding transcriptional regulator SutR	-
JFX59_RS03595 (711310)	711310..712524	+	1215	WP_001307190.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	712564..713829	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T204059 WP_000813794.1 NZ_CP075640:c708143-707967 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T204059 NZ_CP100525:1931396-1931499 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15215.62 Da
• Isoelectric Point: 4.7386

>AT204059 WP_077637634.1 NZ_CP075640:c707921-707505 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKPEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT204059 NZ_CP100525:c1931537-1931266 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• PDB: 6HPB