Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 23120..23645 Replicon plasmid pSTEC2018_166-c
Accession NZ_CP075634
Organism Escherichia coli strain 2018-166

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag JFX80_RS26465 Protein ID WP_001159871.1
Coordinates 23340..23645 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag JFX80_RS26460 Protein ID WP_000813639.1
Coordinates 23120..23338 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX80_RS26440 (JFX80_26455) 18934..19203 - 270 WP_000379710.1 SemiSWEET transporter -
JFX80_RS26445 (JFX80_26460) 19205..20221 - 1017 WP_000583427.1 Gfo/Idh/MocA family oxidoreductase -
JFX80_RS26450 (JFX80_26465) 20221..21051 - 831 WP_016230897.1 HAD-IIB family hydrolase -
JFX80_RS26455 (JFX80_26470) 21035..22261 - 1227 WP_000655924.1 DegT/DnrJ/EryC1/StrS family aminotransferase -
JFX80_RS26460 (JFX80_26475) 23120..23338 + 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
JFX80_RS26465 (JFX80_26480) 23340..23645 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
JFX80_RS26470 (JFX80_26485) 23646..24452 + 807 WP_033806549.1 site-specific integrase -
JFX80_RS26475 (JFX80_26490) 24631..25275 + 645 WP_001144036.1 ParA family protein -
JFX80_RS26480 (JFX80_26495) 25362..25670 + 309 WP_000030204.1 molecular chaperone GroEL -
JFX80_RS26485 (JFX80_26500) 26081..27061 + 981 WP_000688509.1 plasmid segregation protein ParM -
JFX80_RS26490 (JFX80_26505) 27054..27470 + 417 WP_001278818.1 plasmid partitioning/stability family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..119130 119130


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T204047 WP_001159871.1 NZ_CP075634:23340-23645 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T204047 NZ_CP100520:c4640470-4640186 [Klebsiella pneumoniae]
ATGACCTATGAACTGGCGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT204047 WP_000813639.1 NZ_CP075634:23120-23338 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT204047 NZ_CP100520:c4640702-4640460 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References